Demirpence Mehmet, Saytekin Ahmet Murat, Sareyyupoglu Baris, Esendal Omer Memduh
Bacteriological Diagnosis Laboratory, Directorate of Veterinary Department, Nicosia, North Cyprus.
Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey.
Vet Med (Praha). 2022 Aug 22;67(10):497-509. doi: 10.17221/150/2021-VETMED. eCollection 2022 Oct.
In this study, the isolation, biotyping and molecular characterisation of from cattle, sheep and goats in North Cyprus are reported on. A total of 319 raw milk samples obtained from seropositive dairy livestock (190 cattle, 74 sheep and 55 goats) and tissue samples including the liver, spleen and abomasal contents obtained from 32 aborted foetal samples (5 cattle, 18 sheep and 9 goats) were analysed for the presence and characterisation of the agent. was isolated and identified from 90 out of 319 (28.2%) milk and 19 out of 32 (59.4%) foetal samples by conventional bacteriological methods. Identification of all 109 isolates was confirmed by using real-time PCR with genus and species-specific primers. Following the preliminary identification, 27 selected isolates representing various counties and herds were further analysed by conventional methods. Twenty (74.1%) isolates were identified as biovar 1 and seven (25.9%) were identified as biovar 3. The Bruce-ladder multiplex PCR assay revealed that all the isolates were field strains. The results of the present study confirmed the presence of in livestock including the cattle population in North Cyprus. Even though the majority of the samples came from seropositive cattle, was not isolated in the study. The results also revealed the potential public health risk of brucellosis in livestock emphasising the need of implementing strict control and eradication strategies against the disease in animal populations in order to protect human health.
本研究报告了北塞浦路斯牛、羊和山羊中该病菌的分离、生物分型及分子特征。对从血清学阳性的奶牛（190头）、绵羊（74只）和山羊（55只）获得的319份生鲜乳样本，以及从32份流产胎儿样本（5头牛、18只羊和9只山羊）获得的包括肝脏、脾脏和皱胃内容物的组织样本进行分析，以检测该病原体的存在并对其进行特征鉴定。通过传统细菌学方法，从319份乳样本中的90份（28.2%）以及32份胎儿样本中的19份（59.4%）分离并鉴定出了该病菌。使用属和种特异性引物的实时PCR法对所有109株分离株进行了鉴定确认。初步鉴定后，对代表不同县和畜群的27株选定分离株采用传统方法进一步分析。20株（74.1%）分离株被鉴定为生物变种1，7株（25.9%）被鉴定为生物变种3。布鲁斯梯式多重PCR检测显示所有分离株均为田间菌株。本研究结果证实北塞浦路斯包括牛群在内的家畜中存在该病菌。尽管大多数样本来自血清学阳性的牛，但在本研究中未分离出该病菌。研究结果还揭示了家畜布鲁氏菌病对公共卫生的潜在风险，强调需要对动物群体实施严格的疾病控制和根除策略以保护人类健康。
