Multi-view data visualisation manifold learning.

Non-linear dimensionality reduction can be performed by manifold learning approaches, such as stochastic neighbour embedding (SNE), locally linear embedding (LLE) and isometric feature mapping (ISOMAP). These methods aim to produce two or three latent embeddings, primarily to visualise the data in intelligible representations. This manuscript proposes extensions of Student's t-distributed SNE (t-SNE), LLE and ISOMAP, for dimensionality reduction and visualisation of multi-view data. Multi-view data refers to multiple types of data generated from the same samples. The proposed multi-view approaches provide more comprehensible projections of the samples compared to the ones obtained by visualising each data-view separately. Commonly, visualisation is used for identifying underlying patterns within the samples. By incorporating the obtained low-dimensional embeddings from the multi-view manifold approaches into the K-means clustering algorithm, it is shown that clusters of the samples are accurately identified. Through extensive comparisons of novel and existing multi-view manifold learning algorithms on real and synthetic data, the proposed multi-view extension of t-SNE, named multi-SNE, is found to have the best performance, quantified both qualitatively and quantitatively by assessing the clusterings obtained. The applicability of multi-SNE is illustrated by its implementation in the newly developed and challenging multi-omics single-cell data. The aim is to visualise and identify cell heterogeneity and cell types in biological tissues relevant to health and disease. In this application, multi-SNE provides an improved performance over single-view manifold learning approaches and a promising solution for unified clustering of multi-omics single-cell data.