Wang Yabin, Yu Ze, Smith Carlas S, Caneva Sabina
Department of Precision and Microsystems Engineering, Delft University of Technology, Mekelweg 2, 2628 CD, Delft, The Netherlands.
Delft Center for Systems and Control, Delft University of Technology, Mekelweg 2, 2628 CD Delft, Netherlands.
Nano Lett. 2024 Jul 17;24(28):8510-8517. doi: 10.1021/acs.nanolett.4c00673. Epub 2024 Jun 10.
Optical emitters in hexagonal boron nitride (hBN) are promising probes for single-molecule sensing platforms. When engineered in nanoparticle form, they can be integrated as detectors in nanodevices, yet positional control at the nanoscale is lacking. Here we demonstrate the functionalization of DNA origami nanopores with optically active hBN nanoparticles (NPs) with nanometer precision. The NPs are active under three wavelengths of visible illumination and display both stable and blinking emission, enabling their accurate localization by using wide-field optical nanoscopy. Correlative opto-structural characterization reveals deterministic binding of bright, multicolor hBN NPs at the pore rim due to π-π stacking interactions at site-specific locations on the DNA origami. Our work provides a scalable, bottom-up approach toward deterministic assembly of solid-state emitters on arbitrary structural elements based on DNA origami. Such a nanoscale arrangement of optically active components can advance the development of single-molecule platforms, including optical nanopores and nanochannel sensors.
六方氮化硼（hBN）中的光学发射器是单分子传感平台很有前景的探针。当制成纳米颗粒形式时，它们可以作为探测器集成到纳米器件中，但缺乏纳米级的位置控制。在这里，我们展示了具有光学活性的hBN纳米颗粒（NPs）以纳米精度对DNA折纸纳米孔进行功能化。这些纳米颗粒在三种可见光照波长下具有活性，并显示出稳定和闪烁发射，通过使用宽场光学纳米显微镜能够对其进行精确定位。相关的光学结构表征揭示了由于DNA折纸上特定位置的π-π堆积相互作用，明亮的多色hBN纳米颗粒在孔边缘的确定性结合。我们的工作为基于DNA折纸在任意结构元件上确定性组装固态发射器提供了一种可扩展的自下而上的方法。这种光学活性组件的纳米级排列可以推动包括光学纳米孔和纳米通道传感器在内的单分子平台的发展。
