Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
J Transl Med. 2024 Jun 11;22(1):558. doi: 10.1186/s12967-024-05367-5.
The purpose of the study was to evaluate the expression and function of basic leucine zipper ATF-like transcription factor (BATF) in colorectal cancer (CRC), and its correlation with 2-deoxy-2[F]fluoro-D-glucose (F-FDG) positron emission tomography/computed tomography (PET/CT) parameters.
The TIMER database, GEPIA database, TCGA, and GEO database were used to analyze the expression profile of BATF in human cancers. The reverse transcription‑quantitative PCR and western blot analyses were used to evaluate the mRNA level and protein expression in different CRC cell lines. The expression of BATF in SW620 and HCT116 cells was silenced and cell counting kit-8 assays and clonogenic assay were utilized to evaluate the role of BATF in CRC proliferation. The expression of tumor BATF and glucose transporter 1 (GLUT-1) were examined using immunohistochemical tools in 37 CRC patients undergoing preoperative F-FDG PET/CT imaging. The correlation between the PET/CT parameters and immunohistochemical result was evaluated.
In database, BATF was highly expressed in pan-cancer analyses, including CRC, and was associated with poor prognosis in CRC. In vitro, the results showed that knocking down of BATF expression could inhibit the proliferation of SW620 and HCT116 cells. In CRC patients, BATF expression was upregulated in tumor tissues compared with matched para-tumoral tissues, and was related with gender and Ki-67 levels. BATF expression was positively related to GLUT-1 expression and PET/CT parameters, including tumor size, maximum standard uptake value, metabolic tumor volume, and total lesion glycolysis. The multiple logistic analyses showed that SUV was an independent predictor of BATF expression. With 15.96 g/cm as the cutoff, sensitivity was 85.71%, specificity 82.61%, and area-under-the-curve 0.854.
BATF may be an oncogene associated with F-FDG PET/CT parameters in CRC. SUV may be an independent predictor of BATF expression.
本研究旨在评估碱性亮氨酸拉链 ATF 样转录因子（BATF）在结直肠癌（CRC）中的表达和功能，及其与 2-脱氧-2[F]氟-D-葡萄糖（F-FDG）正电子发射断层扫描/计算机断层扫描（PET/CT）参数的相关性。
使用 TIMER 数据库、GEPIA 数据库、TCGA 和 GEO 数据库分析 BATF 在人类癌症中的表达谱。采用逆转录-定量 PCR 和 Western blot 分析评估不同 CRC 细胞系中的 mRNA 水平和蛋白表达。沉默 SW620 和 HCT116 细胞中的 BATF 表达，并用细胞计数试剂盒-8 分析和集落形成实验评估 BATF 在 CRC 增殖中的作用。在接受术前 F-FDG PET/CT 成像的 37 例 CRC 患者中，使用免疫组织化学工具检测肿瘤 BATF 和葡萄糖转运蛋白 1（GLUT-1）的表达。评估 PET/CT 参数与免疫组化结果之间的相关性。
在数据库中，BATF 在泛癌分析中高表达，包括 CRC，并与 CRC 的不良预后相关。在体外，结果表明下调 BATF 表达可抑制 SW620 和 HCT116 细胞的增殖。在 CRC 患者中，肿瘤组织中 BATF 的表达高于配对的肿瘤旁组织，并且与性别和 Ki-67 水平相关。BATF 表达与 GLUT-1 表达和 PET/CT 参数呈正相关，包括肿瘤大小、最大标准摄取值、代谢肿瘤体积和总肿瘤糖酵解。多变量逻辑分析显示，SUV 是 BATF 表达的独立预测因子。以 15.96 g/cm 作为截止值，敏感性为 85.71%，特异性为 82.61%，曲线下面积为 0.854。
BATF 可能是与 CRC 的 F-FDG PET/CT 参数相关的癌基因。SUV 可能是 BATF 表达的独立预测因子。
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