Chaudhari Vishal Sharad, Bose Susmita
W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA.
J Drug Deliv Sci Technol. 2023 Dec;90. doi: 10.1016/j.jddst.2023.105169. Epub 2023 Nov 11.
Natural medicinal compounds (NMCs) can assist effectively in treating bone disorders. NMC release kinetics from a ceramic bone tissue engineering scaffold can be tailored. However, inferior physicochemical properties halt their therapeutic applications and need a carrier system for delivery. We developed a multi-functionalized scaffold to understand the effect of curcumin (Cur) and resveratrol (Rsv) on biological properties. Polycaprolactone (PCL) nanoparticles encapsulated resveratrol in the polymeric matrix. Nanoparticles showed a hydrodynamic diameter of about 180 nm, - 16 mV zeta potential, and up to ~65 % encapsulation efficiency. Scaffolds made of zinc-doped tricalcium phosphate (Zn-TCP) were coated with curcumin followed by either resveratrol (Cur-Rsv) or resveratrol nanoparticles (Cur-Rsv-NP). NMC-loaded scaffolds exhibited a biphasic release pattern over 60 days. Solubility and hydrophobic-hydrophilic interactions affected the NMC release profile. Resveratrol showed rapid release as compared to curcumin. The treated scaffold increased the cell viability of human fetal osteoblast (hFOB) by 1.8-fold as compared to the control. It exhibited a 6-fold increase in cytotoxicity toward osteosarcoma (MG-63) cells as compared to the untreated scaffold. NMCs loaded scaffold effectively inhibited from colonizing over the scaffold. Zinc doping enhanced osteoblast growth and prevented bacterial colony formation. Such design principle provided a direction for developing multi-functionalized calcium phosphate (CaP) scaffolds against bone diseases for orthopedic applications.
天然药用化合物（NMCs）可有效辅助治疗骨疾病。陶瓷骨组织工程支架中NMC的释放动力学可以进行调整。然而，较差的物理化学性质阻碍了它们的治疗应用，并且需要载体系统来进行递送。我们开发了一种多功能支架，以了解姜黄素（Cur）和白藜芦醇（Rsv）对生物学特性的影响。聚己内酯（PCL）纳米颗粒将白藜芦醇包裹在聚合物基质中。纳米颗粒的流体动力学直径约为180 nm，ζ电位为 - 16 mV，包封效率高达约65%。由锌掺杂的磷酸三钙（Zn-TCP）制成的支架先涂覆姜黄素，然后再涂覆白藜芦醇（Cur-Rsv）或白藜芦醇纳米颗粒（Cur-Rsv-NP）。负载NMC的支架在60天内呈现双相释放模式。溶解度和疏水 - 亲水相互作用影响了NMC的释放曲线。与姜黄素相比，白藜芦醇释放迅速。与对照组相比，经处理的支架使人类胎儿成骨细胞（hFOB）的细胞活力提高了1.8倍。与未处理的支架相比，它对骨肉瘤（MG-63）细胞的细胞毒性增加了6倍。负载NMC的支架有效抑制了在支架上的定植。锌掺杂促进了成骨细胞生长并防止了细菌菌落形成。这种设计原则为开发用于骨科应用的抗骨疾病多功能磷酸钙（CaP）支架提供了方向。
