Fan Ruizhi, Xu Teng, Kuang Yuting
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215006, Jiangsu, China.
Department of Gastrointestinal Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Discov Oncol. 2024 Jun 12;15(1):225. doi: 10.1007/s12672-024-01085-y.
Colorectal cancer, which originates from the human colon or rectum, is one of the leading causes of death worldwide. Timely diagnosis and interventional therapy can significantly improve the prognostic survival of colorectal cancer patients, making regular screening and early detection essential.
To investigate the regulatory function of lncRNA CTBP1-DT (CTBP1-DT) on colorectal cancer cells and to assess its diagnostic significance.
A total of 102 patients with colorectal cancer and 92 healthy individuals were selected. The levels of CTBP1-DT and microRNA-30a-5p (miR-30a-5p) in serum and cell samples of the above subjects were compared by RT-qPCR. The effects of CTBP1-DT and miR-30a-5p dysregulation on the biological functions of colorectal cancer cells were analyzed via CCK-8, flow cytometry and Transwell assays. In addition, the ability of CTBP1-DT and miR-30a-5p to early identify colorectal cancer patients was determined through ROC curve.
Serum CTBP1-DT was elevated in patients with colorectal cancer, which was obviously higher than in healthy controls. The expression of serum miR-30a-5p was downregulated in colorectal cancer. Both CTBP1-DT and miR-30a-5p have the value of distinguishing colorectal cancer, and the combined diagnostic ability is higher. Knockdown of CTBP1-DT directly targeted miR-30a-5p to repress cell activity and metastatic ability, whereas deregulation of miR-30a-5p eliminated the above inhibitory effects.
Overexpression of CTBP1-DT has a certain application potential in the diagnosis of colorectal cancer and may be a therapeutic target for colorectal cancer.
结直肠癌起源于人类结肠或直肠，是全球主要的死亡原因之一。及时诊断和介入治疗可显著提高结直肠癌患者的预后生存率，因此定期筛查和早期发现至关重要。
探讨长链非编码RNA CTBP1-DT（CTBP1-DT）对结直肠癌细胞的调控作用，并评估其诊断意义。
选取102例结直肠癌患者和92例健康个体。采用RT-qPCR比较上述受试者血清和细胞样本中CTBP1-DT和微小RNA-30a-5p（miR-30a-5p）的水平。通过CCK-8、流式细胞术和Transwell实验分析CTBP1-DT和miR-30a-5p失调对结直肠癌细胞生物学功能的影响。此外，通过ROC曲线确定CTBP1-DT和miR-30a-5p早期识别结直肠癌患者的能力。
结直肠癌患者血清CTBP1-DT升高，明显高于健康对照组。结直肠癌患者血清miR-30a-5p表达下调。CTBP1-DT和miR-30a-5p均具有区分结直肠癌的价值，联合诊断能力更高。敲低CTBP1-DT直接靶向miR-30a-5p以抑制细胞活性和转移能力，而miR-30a-5p失调则消除了上述抑制作用。
CTBP1-DT的过表达在结直肠癌诊断中具有一定的应用潜力，可能是结直肠癌的治疗靶点。
