Fink A, Shani A, Weisman Z, Wechsler U, Pfeffermann R, Harpaz N, Eitan S, Bentwich Z
Immunol Lett. 1985;9(2-3):143-7. doi: 10.1016/0165-2478(85)90025-2.
Immunity to colorectal cancer antigen was tested by the tube leukocyte adherence inhibition (LAI) assay, using spent medium of human carcinoma cell lines as a source of antigen. The assay was positive in 18 out of 43 patients (41%) with colorectal tumor in comparison to only 2 out of 34 (5.8%) of colorectal cancer patients clinically free of disease, and to 4 out of 150 (2.6%) healthy subjects. The frequency of positive results correlated negatively with the tumor burden, 16 out of 29 (55.1%) patients with Dukes' A, B and C being positive compared to only 2 out of 14 (14.2%) patients with Dukes' stage D disease. Addition of PGE2 enhanced the sensitivity of the assay without affecting its specificity. The number of positive assays increased from 18 to 32 (41.8% to 74.4%) in the whole group of 43 colorectal cancer patients without altering significantly the frequency of positive results in the control group. The results with all groups of patients were influenced similarly by the addition of prostaglandins, the frequency of positive assays increasing from 55.1% to 82.7% and from 14.1% to 57.1% in early and advanced disease, respectively. These results lend further support to the value of the LAI assay in diagnosis and monitoring of the immune response in human colorectal cancer.
采用人癌细胞系的用过的培养基作为抗原来源，通过试管白细胞黏附抑制（LAI）试验检测对结直肠癌抗原的免疫反应。43例结直肠癌患者中有18例（41%）该试验呈阳性，相比之下，34例临床无病的结直肠癌患者中只有2例（5.8%）呈阳性，150例健康受试者中只有4例（2.6%）呈阳性。阳性结果的频率与肿瘤负荷呈负相关，29例 Dukes' A、B 和 C 期患者中有16例（55.1%）呈阳性，而 Dukes' D 期疾病的14例患者中只有2例（14.2%）呈阳性。添加前列腺素E2可提高该试验的敏感性，而不影响其特异性。在43例结直肠癌患者的整个队列中，阳性试验的数量从18例增加到32例（从41.8%增加到74.4%），而对照组阳性结果的频率没有显著改变。添加前列腺素对所有患者组的结果影响相似，早期和晚期疾病中阳性试验的频率分别从55.1%增加到82.7%和从14.1%增加到57.1%。这些结果进一步支持了LAI试验在人类结直肠癌免疫反应诊断和监测中的价值。
