College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Int J Biol Macromol. 2019 May 1;128:858-869. doi: 10.1016/j.ijbiomac.2019.01.214. Epub 2019 Feb 1.
Lepista nuda has high nutritional and medicinal value, especially has high antioxidant, antitumor and antiviral activities. Based on results of single factor experiment, response surface methodology was used to optimize the extraction conditions of polysaccharides (LNP). Macroporous resin D301 was used to decolorization, and its technological conditions were determined by orthogonal experiment. In addition, two novel polysaccharides (LNP-1, LNP-2) were purified by DEAE Cellulose-52 and Sephadex G-150 chromatography. The molecular weight of LNP-1 is 11,703Da, mainly composed of mannose, glucose, galactose, xylose, arabinose and fucose, while the molecular weight of LNP-2 is 13,369Da, mainly composed of mannose, glucose, galactose, arabinose and fucose. The polysaccharide contents of crude LNP, LNP-1 and LNP-2 were 70.60%, 87.71% and 81.20% respectively, and the protein contents were 1.72%, 0.97% and 0.68% respectively. Their sulfuric contents were 3.39%, 5.02% and 8.64%, and uronic acid contents were 3.66%, 5.56% and 6.80%, respectively. Further studies showed that their ability to chelate iron ions, scavenge DPPH (1,1‑diphenyl‑2‑picrylhydrazyl) free radicals and scavenge superoxide anion radicals were 70.09%, 55.94% and 36.64% respectively. They showed strong ability to scavenge free radicals in a concentration-dependent manner. LNP is expected to be developed as a new efficacy factor in the food industry.
裸盖菇素具有很高的营养价值和药用价值，特别是具有很高的抗氧化、抗肿瘤和抗病毒活性。基于单因素实验结果，采用响应面法优化了多糖（LNP）的提取条件。大孔树脂 D301 用于脱色，并通过正交实验确定其工艺条件。此外，通过 DEAE 纤维素-52 和 Sephadex G-150 层析法纯化了两种新型多糖（LNP-1、LNP-2）。LNP-1 的分子量为 11703Da，主要由甘露糖、葡萄糖、半乳糖、木糖、阿拉伯糖和岩藻糖组成，而 LNP-2 的分子量为 13369Da，主要由甘露糖、葡萄糖、半乳糖、阿拉伯糖和岩藻糖组成。粗 LNP、LNP-1 和 LNP-2 的多糖含量分别为 70.60%、87.71%和 81.20%，蛋白质含量分别为 1.72%、0.97%和 0.68%。它们的硫酸基含量分别为 3.39%、5.02%和 8.64%，糖醛酸含量分别为 3.66%、5.56%和 6.80%。进一步研究表明，它们螯合铁离子、清除 DPPH（1,1-二苯基-2-苦基肼）自由基和清除超氧阴离子自由基的能力分别为 70.09%、55.94%和 36.64%。它们在浓度依赖性方式下表现出强大的清除自由基能力。LNP 有望作为食品工业中的一种新的功效因子得到开发。
