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sp. nov.,一种从. 的肠道内容物中分离出来的新型细菌。

sp. nov., a novel bacterium isolated from the gut content of .

机构信息

Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.

Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006419.

Abstract

A Gram-stain-positive, aerobic bacterium, designated as YPD9-1, was isolated from the gut contents of a spotty belly greenling, , collected near Dokdo island, South Korea. The rod-shaped cells were oxidase-positive, and catalase-negative. The major cellular fatty acids were anteiso-C, iso-C, C, iso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 47.6 mol% and the predominant respiratory quinone was menaquinone MK-7. The 16S rRNA gene sequence of YPD9-1 showed low sequence similarities to species of the genus , Gsoil 1138 (97.21 % of sequence similarity), CJ25 (97.12 %) and JJ-42 (96.89 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that YPD9-1 formed a distinct branch among other species of the genus . The digital DNA-DNA hybridisation, average nucleotide identity, and average amino acid identity values between YPD9-1 and the related species were in the ranges of 15.3-16.2 %, 74.1-78.4 %, and 71.1-71.9 %, respectively, which are below the species cutoff values. On the basis of the results of the polyphasic analysis, we conclude that strain YPD9-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is YPD9-1 (=KCTC 43424 =LMG 32988).

摘要

一株革兰氏阳性、需氧细菌,被命名为 YPD9-1,从韩国独岛附近采集的斑点腹鳕鱼的肠道内容物中分离得到。该杆状细胞氧化酶阳性,过氧化氢酶阴性。主要细胞脂肪酸为 anteiso-C、iso-C、C、iso-C 和 iso-C。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的脂类。DNA G+C 含量为 47.6 mol%,主要呼吸醌为menaquinone MK-7。YPD9-1 的 16S rRNA 基因序列与属内的种,Gsoil 1138(97.21%序列相似性)、CJ25(97.12%)和 JJ-42(96.89%)的序列相似性较低。基于 16S rRNA 基因序列的系统发育分析结果表明,YPD9-1 在属内的其他种中形成了一个独特的分支。YPD9-1 与相关种之间的数字 DNA-DNA 杂交、平均核苷酸同一性和平均氨基酸同一性值分别在 15.3-16.2%、74.1-78.4%和 71.1-71.9%之间,均低于种的分界值。基于多相分析的结果,我们认为菌株 YPD9-1 代表了属的一个新种,因此提议使用 sp. nov. 命名。的模式菌株是 YPD9-1(=KCTC 43424=LMG 32988)。

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