Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006419.
A Gram-stain-positive, aerobic bacterium, designated as YPD9-1, was isolated from the gut contents of a spotty belly greenling, , collected near Dokdo island, South Korea. The rod-shaped cells were oxidase-positive, and catalase-negative. The major cellular fatty acids were anteiso-C, iso-C, C, iso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 47.6 mol% and the predominant respiratory quinone was menaquinone MK-7. The 16S rRNA gene sequence of YPD9-1 showed low sequence similarities to species of the genus , Gsoil 1138 (97.21 % of sequence similarity), CJ25 (97.12 %) and JJ-42 (96.89 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that YPD9-1 formed a distinct branch among other species of the genus . The digital DNA-DNA hybridisation, average nucleotide identity, and average amino acid identity values between YPD9-1 and the related species were in the ranges of 15.3-16.2 %, 74.1-78.4 %, and 71.1-71.9 %, respectively, which are below the species cutoff values. On the basis of the results of the polyphasic analysis, we conclude that strain YPD9-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is YPD9-1 (=KCTC 43424 =LMG 32988).
一株革兰氏阳性、需氧细菌，被命名为 YPD9-1，从韩国独岛附近采集的斑点腹鳕鱼的肠道内容物中分离得到。该杆状细胞氧化酶阳性，过氧化氢酶阴性。主要细胞脂肪酸为 anteiso-C、iso-C、C、iso-C 和 iso-C。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的脂类。DNA G+C 含量为 47.6 mol%，主要呼吸醌为menaquinone MK-7。YPD9-1 的 16S rRNA 基因序列与属内的种，Gsoil 1138（97.21%序列相似性）、CJ25（97.12%）和 JJ-42（96.89%）的序列相似性较低。基于 16S rRNA 基因序列的系统发育分析结果表明，YPD9-1 在属内的其他种中形成了一个独特的分支。YPD9-1 与相关种之间的数字 DNA-DNA 杂交、平均核苷酸同一性和平均氨基酸同一性值分别在 15.3-16.2%、74.1-78.4%和 71.1-71.9%之间，均低于种的分界值。基于多相分析的结果，我们认为菌株 YPD9-1 代表了属的一个新种，因此提议使用 sp. nov. 命名。的模式菌株是 YPD9-1（=KCTC 43424=LMG 32988）。
