State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Int J Mol Sci. 2023 Oct 23;24(20):15459. doi: 10.3390/ijms242015459.
, commonly known as Chinese Toon, is a plant species that possesses noteworthy value as a tree and vegetable. Its tender young buds exhibit a diverse range of colors, primarily determined by the presence and composition of anthocyanins and flavonoids. However, the underlying mechanisms of anthocyanin biosynthesis in have been rarely reported. To explore the related genes and metabolites associated with composition of leaf color, we conducted an analysis of the transcriptome and metabolome of five distinct clones. The results showed that differentially expressed genes and metabolites involved in anthocyanin biosynthesis pathway were mainly enriched. A conjoint analysis of transcripts and metabolites was carried out in JFC (red) and LFC (green), resulting in the identification of 510 genes and 23 anthocyanin-related metabolites with a positive correlation coefficient greater than 0.8. Among these genes and metabolites, 23 transcription factors and phytohormone-related genes showed strong coefficients with 13 anthocyanin derivates, which mainly belonged to the stable types of delphinidin, cyanidin, peonidin. The core derivative was found to be Cyanidin-3-O-arabinoside, which was present in JFC at 520.93 times the abundance compared to LFC. Additionally, the regulatory network and relative expression levels of genes revealed that the structural genes , , and might be directly or indirectly regulated by the transcription factors SOC1 (MADS-box), CPC (MYB), and bHLH162 (bHLH) to control the accumulation of anthocyanin. The expression of these genes was significantly higher in red clones compared to green clones. Furthermore, RNA-seq results accurately reflected the true expression levels of genes. Overall, this study provides a foundation for future research aimed at manipulating anthocyanin biosynthesis to improve plant coloration or to derive human health benefits.
香椿，俗称香椿，是一种具有树木和蔬菜双重价值的植物。其幼嫩的芽呈现出丰富多样的颜色，主要取决于花色素苷和类黄酮的存在和组成。然而，香椿中花色素苷生物合成的机制很少有报道。为了探讨与叶片颜色组成相关的基因和代谢物，我们对 5 个不同香椿克隆体的转录组和代谢组进行了分析。结果表明，差异表达基因和代谢物主要富集在花色素苷生物合成途径中。对 JFC（红色）和 LFC（绿色）中的转录本和代谢物进行联合分析，鉴定出 510 个与花色素苷相关的基因和 23 种代谢物，其转录本和代谢物的相关系数均大于 0.8。在这些基因和代谢物中，23 个转录因子和植物激素相关基因与 13 种花色素苷衍生物表现出较强的相关性，主要属于稳定型的飞燕草素、矢车菊素、芍药素。核心衍生物为矢车菊-3-O-阿拉伯糖苷，在 JFC 中的丰度比 LFC 高 520.93 倍。此外，基因的调控网络和相对表达水平表明，结构基因、和可能直接或间接受到转录因子 SOC1（MADS-box）、CPC（MYB）和 bHLH162（bHLH）的调控，从而控制花色素苷的积累。这些基因在红色克隆体中的表达明显高于绿色克隆体。此外，RNA-seq 结果准确反映了基因的真实表达水平。总的来说，本研究为未来操纵花色素苷生物合成以改善植物着色或获得人类健康益处的研究提供了基础。
