You Qiaomu, Ren Yanjun, Li Jiaotong, Zeng Guangnian, Luo Xiongfei, Zheng Chunying, Tang Zhonghua
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongiang Province & School of Life Sciences, Heilongiiang University, Harbin 150080, China.
Molecules. 2025 Jan 18;30(2):397. doi: 10.3390/molecules30020397.
(ASC) contains a variety of bioactive compounds and serves as an important traditional Chinese medicinal resource. However, its prolonged growth cycle and reliance on wild populations limit its practical use. To explore the potential of (ASF) as an alternative, this study focused on optimizing the extraction process and assessing the bioactivity of stem extracts. The Analytic Hierarchy Process-Entropy Weight Method (AHP-EWM) was applied to comprehensively score five key active compounds in ASF stems, establishing a benchmark for evaluating extraction efficiency. Ultrasound-assisted enzymatic extraction (UAEE) was employed, and orthogonal and response surface experiments were conducted to refine the extraction parameters. The optimal conditions identified were an ultrasound temperature of 59 °C, a duration of 57 min, an ethanol concentration of 61%, and a liquid-to-material ratio of 39:1, resulting in an AHP-EWM composite score of 94.76. Comparative analysis of antibacterial and antioxidant activities revealed that ASC extracts exhibited superior antibacterial effects, while ASF extracts demonstrated enhanced antioxidant properties. These findings support the partial replacement of ASC with ASF, contributing to the conservation of wild resources and expanding the application of ASF in medicinal development.
（某植物）（ASC）含有多种生物活性化合物，是一种重要的传统中药资源。然而，其生长周期长且依赖野生种群限制了其实际应用。为探索（某植物）（ASF）作为替代品的潜力，本研究聚焦于优化提取工艺并评估茎提取物的生物活性。应用层次分析法-熵权法（AHP-EWM）对ASF茎中的五种关键活性化合物进行综合评分，为评估提取效率建立基准。采用超声辅助酶提取法（UAEE），并进行正交试验和响应面试验以优化提取参数。确定的最佳条件为超声温度59℃、时间57分钟、乙醇浓度61%、液料比39:1，AHP-EWM综合评分为94.76。抗菌和抗氧化活性的对比分析表明，ASC提取物表现出更强的抗菌作用，而ASF提取物具有更强的抗氧化性能。这些发现支持用ASF部分替代ASC，有助于野生资源保护并扩大ASF在药物开发中的应用。
