Ertuğrul Uygun Hilmiye Deniz, Odaci Dilek
Center for Fabrication and Application of Electronic Materials, Dokuz Eylül University, Buca, İzmir 35220, Türkiye.
Faculty of Science, Department of Biochemistry, Ege University, Bornova, İzmir 35040, Türkiye.
ACS Omega. 2024 May 24;9(23):25172-25180. doi: 10.1021/acsomega.4c02738. eCollection 2024 Jun 11.
This study introduces a novel biosensing approach for the detection of pathogen DNA in breast milk utilizing single carbon fiber electrodes (SCFE) enhanced with MXene nanomaterial layers. The primary innovation lies in the modification of SCFE with MXenes to increase the electrode's surface area, followed by surface activation for the immobilization of dCas9-sgRNA complexes. This modification aims to leverage the unique properties of MXenes and the selective binding capability of the CRISPR technology for efficient and specific pathogen detection. Electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) analyses were employed to characterize the electrode modifications and the immobilization process, demonstrating the successful enhancement of biosensor performance. This study further optimized the chronoimpedimetric detection method to achieve rapid, sensitive, and selective detection of (SAu) DNA in breast milk, with a notable detection time of 60 s in real samples. The biosensor demonstrated high selectivity and sensitivity, with a linear detection range between 50 and 6000 fM and a limit of detection (LOD) of 14.5 fM. The reproducibility and stability of the biosensor were also confirmed through multiple tests, showing promising potential for clinical and public health applications.
本研究介绍了一种利用涂覆有MXene纳米材料层的单碳纤维电极（SCFE）检测母乳中病原体DNA的新型生物传感方法。主要创新点在于用MXenes修饰SCFE以增加电极表面积，随后进行表面活化以固定dCas9-sgRNA复合物。这种修饰旨在利用MXenes的独特性质以及CRISPR技术的选择性结合能力，实现高效、特异性的病原体检测。采用电化学阻抗谱（EIS）和扫描电子显微镜（SEM）分析来表征电极修饰和固定过程，证明生物传感器性能得到成功提升。本研究进一步优化了计时阻抗检测方法，以实现对母乳中金黄色葡萄球菌（SAu）DNA的快速、灵敏和选择性检测，实际样品中的显著检测时间为60秒。该生物传感器具有高选择性和灵敏度，线性检测范围在50至6000 fM之间，检测限（LOD）为14.5 fM。通过多次测试还证实了该生物传感器的重现性和稳定性，显示出在临床和公共卫生应用中的广阔前景。
