Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan.
College of Bioscience and Biotechnology, Chubu University, Aichi 487-8501, Japan.
Molecules. 2024 Jun 3;29(11):2632. doi: 10.3390/molecules29112632.
Cancer cells have higher heat sensitivity compared to normal cells; therefore, hyperthermia is a promising approach for cancer therapy because of its ability to selectively kill cancer cells by heating them. However, the specific and rapid heating of tumor tissues remains challenging. This study investigated the potential of magnetic nanoparticles (MNPs) modified with tumor-homing peptides (THPs), specifically PL1 and PL3, for tumor-specific magnetic hyperthermia therapy. The synthesis of THP-modified MNPs involved the attachment of PL1 and PL3 peptides to the surface of the MNPs, which facilitated enhanced tumor cell binding and internalization. Cell specificity studies revealed an increased uptake of PL1- and PL3-MNPs by tumor cells compared to unmodified MNPs, indicating their potential for targeted delivery. In vitro hyperthermia experiments demonstrated the efficacy of PL3-MNPs in inducing tumor cell death when exposed to an alternating magnetic field (AMF). Even without exposure to an AMF, an additional ferroptotic pathway was suggested to be mediated by the nanoparticles. Thus, this study suggests that THP-modified MNPs, particularly PL3-MNPs, hold promise as a targeted approach for tumor-specific magnetic hyperthermia therapy.
癌细胞比正常细胞对热更敏感；因此，热疗是一种很有前途的癌症治疗方法，因为它能够通过加热选择性地杀死癌细胞。然而，肿瘤组织的特异性和快速加热仍然具有挑战性。本研究探讨了经肿瘤归巢肽（THP）修饰的磁性纳米颗粒（MNPs），特别是 PL1 和 PL3，用于肿瘤特异性磁热疗的潜力。THP 修饰的 MNPs 的合成涉及将 PL1 和 PL3 肽附着到 MNPs 的表面，这促进了增强的肿瘤细胞结合和内化。细胞特异性研究表明，与未修饰的 MNPs 相比，PL1 和 PL3-MNPs 被肿瘤细胞更多地摄取，表明它们具有靶向递送的潜力。体外热疗实验表明，PL3-MNPs 在暴露于交变磁场（AMF）时能有效诱导肿瘤细胞死亡。即使不暴露于 AMF，也有研究表明纳米颗粒介导了一种额外的铁死亡途径。因此，本研究表明，THP 修饰的 MNPs，特别是 PL3-MNPs，有望成为肿瘤特异性磁热疗的一种靶向方法。
