sp. nov.，分离自冰川。

sp. nov., isolated from a glacier.

机构信息

China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006433.

DOI:10.1099/ijsem.0.006433

PMID:38896461

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11261710/

Abstract

A Gram-stain-positive, rod-shaped bacterium, designated as HLT2-17, was isolated from soil sample taken from the Hailuogou glacier in Sichuan province, PR China. Strain HLT2-17 was capable of growing at 4-25°C and in NaCl concentrations ranging from 0 to 2% (w/v). The highest level of 16S rRNA gene sequence similarity was observed with M0-14 (98.3 %) and LRZ-2 (98.2 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain HLT2-17 and its closest relatives, M0-14 and LRZ-2, were 80.0-84.0 % and 23.3-27.7 %, respectively. Phylogenomic analysis indicated that strain HLT2-17 clustered together with strains M0-14 and LRZ-2. Strain HLT2-17 contained C and anteiso-C as the major fatty acids, and MK-9(H) as the menaquinone. Therefore, based on a polyphasic approach, we propose that strain HLT2-17 (=CGMCC 1.11116= NBRC 110443) represents a novel species of the genus and suggest the name sp. nov.

摘要

一株革兰氏阳性、杆状细菌，被命名为 HLT2-17，是从中国四川省海螺沟冰川的土壤样本中分离出来的。菌株 HLT2-17 能够在 4-25°C 和 0-2%（w/v）的 NaCl 浓度下生长。与 M0-14（98.3%）和 LRZ-2（98.2%）相比，菌株 HLT2-17 的 16S rRNA 基因序列相似度最高。菌株 HLT2-17 与其最接近的亲缘关系菌株 M0-14 和 LRZ-2 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 80.0-84.0%和 23.3-27.7%。系统发育基因组分析表明，菌株 HLT2-17 与 M0-14 和 LRZ-2 菌株聚在一起。菌株 HLT2-17 含有 C 和 anteiso-C 作为主要脂肪酸，以及 MK-9(H) 作为menaquinone。因此，基于多相方法，我们建议菌株 HLT2-17（=CGMCC 1.11116= NBRC 110443）代表一个新的属种，并建议使用名称 sp. nov.。