China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Experimental School of Beihang University, Beijing 100083, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3276-3280. doi: 10.1099/ijsem.0.003620.
A psychrophilic, Gram-stain-positive, rod-shaped bacterium, designated Hh39, was isolated from Xinjiang No. 1 glacier in PR China. Strain Hh39 was catalase-positive, oxidase-negative and could grow at 0-18 °C, pH 6.0-11.0 and in the presence of 0-2.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Hh39 belonged to the genus . The highest level of 16S rRNA gene sequence similarities were found to the type strains of (99.01 %), (98.90 %), (98.90 %) and (98.75 %). However, the low average nucleotide identity (80.65-81.89 %) and digital DNA-DNA hybridization values (22.1-23.8 %) between strain Hh39 and its four closest relatives indicated that it represents a novel species of the genus . The predominant fatty acids were anteiso-C, anteiso-C, iso-C and anteiso-C. The major menaquinone was MK-10. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unidentified lipid and one unidentified glycolipid. On the basis of results of phenotypic, genotypic and phylogenetic analyses, a novel species, sp. nov., is proposed, with Hh39 (=NBRC 107884=CGMCC 1.11212) as the type strain.
一株嗜冷、革兰氏阳性、杆状细菌，被命名为 Hh39，从中国新疆 1 号冰川中分离得到。菌株 Hh39 过氧化氢酶阳性，氧化酶阴性，可以在 0-18°C、pH 值 6.0-11.0 和 0-2.5%（w/v）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 Hh39 属于 属。与该属的 （99.01%）、 （98.90%）、 （98.90%）和 （98.75%）的模式菌株的 16S rRNA 基因序列相似度最高。然而，菌株 Hh39 与它的四个最近亲缘种之间的平均核苷酸同一性（80.65-81.89%）和数字 DNA-DNA 杂交值（22.1-23.8%）较低，表明它代表了 属的一个新种。主要脂肪酸为 anteiso-C、anteiso-C、iso-C 和 anteiso-C。主要的menaquinone 是 MK-10。极性脂为双磷脂酰甘油、磷脂酰甘油、一种未鉴定的脂和一种未鉴定的糖脂。基于表型、基因型和系统发育分析的结果，提出了一个新的物种，即 sp. nov.，以 Hh39（=NBRC 107884=CGMCC 1.11212）作为模式菌株。
