Department of Physics, University of California, Berkeley, CA, USA.
School of Mechanical Science and Engineering - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, China.
Nat Methods. 2024 Jul;21(7):1298-1305. doi: 10.1038/s41592-024-02309-3. Epub 2024 Jun 19.
Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.
在体突触传递的容积成像是需要高空间分辨率和高时间分辨率的。我们通过对双光子荧光激发光的波前整形，开发了贝塞尔液滴焦点，用于高对比度和高分辨率的突触容积成像。将我们的方法应用于谷氨酸释放的成像，我们在活体中展示了超过 1000 个突触/体素和超过 500 个神经元/树突棘的高吞吐量的兴奋性输入映射，并揭示了在小鼠初级视觉皮层中以前看不见的功能突触组织的特征。
