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给猪投喂刚地弓形虫卵囊后组织感染与血清学结果的相关性

Correlation of tissue infection and serologic findings in pigs fed Toxoplasma gondii oocysts.

作者信息

Prickett M D, Dreesen D W, Waltman W D, Blue J L, Brown J

出版信息

Am J Vet Res. 1985 May;46(5):1130-2.

PMID:3890633
Abstract

Each of thirteen 6-week-old pigs was inoculated per os with 10,000 sporulated oocysts of Toxoplasma gondii. By postinoculation day (PID) 13, pigs were seropositive by the indirect fluorescent antibody test. Beginning on PID 13 and every 7 days thereafter through PID 97, 1 pig was killed and 6 tissues were examined for T gondii. Of the 13 pigs, 11 were infected, including the 1st pig killed on PID 13, although none of the pigs had gross lesions of toxoplasmosis. Tissues harboring T gondii most frequently were the heart and brain; organisms were detected less frequently in the longissimus muscles, diaphragm, and liver. Toxoplasma gondii was not detected in the bronchial lymph nodes. There was good correlation between antibody and presence of T gondii in these pigs. One additional pig, maintained as a noninfected control, remained seronegative and had no evidence of infection when killed on PID 97.

摘要

13头6周龄仔猪每头经口接种10000个刚地弓形虫孢子化卵囊。到接种后第13天(PID 13),通过间接荧光抗体试验检测,仔猪血清呈阳性。从PID 13开始,此后每隔7天直至PID 97,处死1头猪,并对6种组织进行刚地弓形虫检测。13头猪中,11头被感染,包括在PID 13处死的第1头猪,尽管这些猪均无弓形虫病的肉眼可见病变。携带刚地弓形虫的组织最常见的是心脏和大脑;在背最长肌、膈肌和肝脏中检测到病原体的频率较低。在支气管淋巴结中未检测到刚地弓形虫。这些猪的抗体与刚地弓形虫的存在之间存在良好的相关性。另外1头作为未感染对照饲养的猪,在PID 97处死时血清仍为阴性,且无感染迹象。

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