经口感染卵囊的马属动物组织中包囊型刚地弓形虫的持续性

Persistence of encysted Toxoplasma gondii in tissues of equids fed oocysts.

作者信息

Dubey J P

出版信息

Am J Vet Res. 1985 Aug;46(8):1753-4.

Abstract

Thirteen 6-month- to 13-year-old equids were each inoculated orally with 10,000 infective Toxoplasma gondii oocysts. The equids remained clinically normal. Equids were euthanatized on postinoculation days 33, 45, 90, 104, 120, 140, 162, 183, 197, 204, 211, 294, and 476. Their tissues were bioassayed for viable oocysts by feeding muscles to Toxoplasma-free cats and by inoculation of pepsin digests of 13 organs into mice. Using mouse inoculation, T gondii was recovered from tissues of the 9 equids euthanatized on each of postinoculation days 33, 45, 120, 140, 183, 197, 211, 294, and 476. Toxoplasma gondii was isolated from the tongue and intestines of 4 equids, from the heart of 3 equids, from thigh muscles, brain, and heart of 2 equids, and from the eye, lungs, and spinal cord of 1 equid. Muscles from 9 of 13 equids were infectious to cats, as evidenced by feline shedding of oocysts. Results indicate that T gondii can persist in edible tissues of living equids up to 476 days and that non-cooked horsemeat should not be fed to cats or eaten by human beings.

摘要

13匹6个月至13岁的马科动物每匹经口接种10000个感染性刚地弓形虫卵囊。这些马科动物在临床上保持正常。在接种后的第33、45、90、104、120、140、162、183、197、204、211、294和476天对这些马科动物实施安乐死。通过将肌肉喂给无弓形虫的猫以及将13个器官的胃蛋白酶消化物接种到小鼠体内，对它们的组织进行活卵囊生物测定。通过小鼠接种，在接种后第33、45、120、140、183、197、211、294和476天实施安乐死的9匹马科动物的组织中检测到了刚地弓形虫。在4匹马科动物的舌头和肠道、3匹马科动物的心脏、2匹马科动物的大腿肌肉、大脑和心脏以及1匹马科动物的眼睛、肺和脊髓中分离出了刚地弓形虫。13匹马科动物中有9匹的肌肉对猫具有感染性，猫排出卵囊证明了这一点。结果表明，刚地弓形虫可在存活马科动物的可食用组织中持续存在长达476天，未煮熟的马肉不应喂给猫或供人类食用。