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马静止期子宫内膜微生物组的宏基因组特征。

Metagenomic characterization of the equine endometrial microbiome during anestrus.

机构信息

Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA.

Department of Population Health and Reproduction (PHR), School of Veterinary Medicine, UCDavis, Davis, CA, 95616, USA; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, UGent, Merelbeke, 9820, Belgium.

出版信息

J Equine Vet Sci. 2024 Sep;140:105134. doi: 10.1016/j.jevs.2024.105134. Epub 2024 Jun 21.

Abstract

The equine uterus is highly interrogated during estrus prior to breeding and establishing pregnancy. Many studies in mares have been performed during estrus under the influence of high estrogen concentrations, including the equine estrual microbiome. To date, it is unknown how the uterine microbiome of the mare is influenced by cyclicity; while, the equine vaginal microbiome is stable throughout the estrous cycle. We hypothesized that differences would exist between the equine endometrial microbiome of mares in estrus and anestrus. The aim of this study was two-fold: to characterize the resident endometrial microbiome of healthy mares during anestrus and to compare this with estrus. Double-guarded endometrial swabs were taken from healthy mares during estrus (n = 16) and in the following non-breeding season during anestrus (n = 8). Microbial population was identified using 16S rRNA sequencing. Our results suggest that the equine uterine microbiome in estrus has a low diversity and low richness, while during anestrus, a higher diversity and higher richness were seen compared to estrus. Despite this difference, both the estrus and anestrus endometrial microbiome were dominated by Proteobacteria, Firmicutes, and Bacteroidota. The composition of the microbial community between anestrus and estrus was significantly different. This may be explained by the difference in the composition of the endometrial immune milieu based on the stage of the cycle. Further research investigating the function of the equine endometrial microbiome and dynamics changes within the uterine environment is required.

摘要

马的子宫在发情期之前进行繁殖和妊娠建立时会受到高度检查。许多关于发情期母马的研究都是在高雌激素浓度的影响下进行的,包括马的发情期微生物组。迄今为止,尚不清楚马的子宫微生物组如何受到周期性的影响;而马的阴道微生物组在整个发情周期中是稳定的。我们假设发情期和非发情期母马的子宫内膜微生物组之间会存在差异。本研究的目的有两个:一是描述非发情期健康母马的常驻子宫内膜微生物组,并将其与发情期进行比较。在发情期(n=16)和随后的非繁殖季节(n=8)期间,从健康母马的子宫内采集双层保护的子宫内膜拭子。使用 16S rRNA 测序鉴定微生物群。我们的研究结果表明,发情期马的子宫微生物组多样性低、丰富度低,而非发情期则与发情期相比,多样性高、丰富度高。尽管存在这种差异,发情期和非发情期的子宫内膜微生物组都以变形菌门、厚壁菌门和拟杆菌门为主。非发情期和发情期之间的微生物群落组成存在显著差异。这可能是由于根据周期阶段,子宫内膜免疫环境的组成不同所致。需要进一步研究马的子宫内膜微生物组的功能以及子宫内环境的动态变化。

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