Nanda Sitansu Sekhar, Kim Danyeong, Yang Hyewon, An Seong Soo A, Yi Dong Kee
Department of Chemistry, Myongji University, Yongin 17058, Republic of Korea.
Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam 13120, Republic of Korea.
Nanomaterials (Basel). 2024 Jun 15;14(12):1033. doi: 10.3390/nano14121033.
Rapid advancements in nanotechnology have expanded its applications and synergistic impact on modern nanosystems. The comprehensive assessment of nanomaterials' safety for human exposure has become crucial and heightened. In addition to the characterization of cell proliferation and apoptosis, probing the implication of autophagy is vital for understanding the ramification of nanomaterials. Hence, HEK-293 kidney cells were employed to understand the changes in induction and perturbation of autophagy in cells by iron oxide (FeO) and silica (SiO) nanoparticles. Interestingly, FeO worked as a potent modulator of the autophagy process through its catalytic performance, which can develop better than that of SiO nanoparticles mechanism, stressing their therapeutic implication in the understanding of cell behaviors. The quantification of reactive oxygen species (ROS) was measured along with the process of autophagy during cell growth. This modulated autophagy will help in cell fate determination in complementary therapy for disease treatment, provide a clinical strategy for future study.
纳米技术的快速发展扩大了其应用范围以及对现代纳米系统的协同影响。对纳米材料人体暴露安全性的全面评估变得至关重要且日益受到关注。除了对细胞增殖和凋亡进行表征外，探究自噬的影响对于理解纳米材料的后果至关重要。因此，采用人胚肾293（HEK - 293）细胞来了解氧化铁（FeO）和二氧化硅（SiO）纳米颗粒对细胞自噬诱导和扰动的变化。有趣的是，FeO通过其催化性能作为自噬过程的有效调节剂，其作用机制比SiO纳米颗粒更好，强调了它们在理解细胞行为方面的治疗意义。在细胞生长过程中，伴随着自噬过程对活性氧（ROS）进行了定量测定。这种调节的自噬将有助于在疾病治疗的辅助治疗中确定细胞命运，为未来研究提供临床策略。
