State Research Center of Virology and Biotechnology "Vector", Koltsovo 630559, Novosibirsk Region, Russia.
Viruses. 2024 Jun 4;16(6):909. doi: 10.3390/v16060909.
In this study, we investigated the features of co-infection with SARS-CoV-2 and the enterovirus vaccine strain LEV8 of coxsackievirus A7 or enterovirus A71 for Vero E6 cells and Syrian hamsters. The investigation of co-infection with SARS-CoV-2 and LEV-8 or EV-A71 in the cell model showed that a competitive inhibitory effect for these viruses was especially significant against SARS-CoV-2. Pre-infection with enteroviruses in the animals caused more than a 100-fold decrease in the levels of SARS-CoV-2 virus replication in the respiratory tract and more rapid clearance of infectious SARS-CoV-2 from the lower respiratory tract. Co-infection with SARS-CoV-2 and LEV-8 or EV-A71 also reduced the severity of clinical manifestations of the SARS-CoV-2 infection in the animals. Additionally, the histological data illustrated that co-infection with strain LEV8 of coxsackievirus A7 decreased the level of pathological changes induced by SARS-CoV-2 in the lungs. Research into the chemokine/cytokine profile demonstrated that the studied enteroviruses efficiently triggered this part of the antiviral immune response, which is associated with the significant inhibition of SARS-CoV-2 infection. These results demonstrate that there is significant viral interference between the studied strain LEV-8 of coxsackievirus A7 or enterovirus A71 and SARS-CoV-2 in vitro and in vivo.
在这项研究中，我们研究了 SARS-CoV-2 与柯萨奇病毒 A7 的肠道病毒疫苗株 LEV8 或肠道病毒 A71 共同感染 Vero E6 细胞和叙利亚仓鼠的特征。在细胞模型中研究 SARS-CoV-2 与 LEV-8 或 EV-A71 的共同感染表明，这些病毒对 SARS-CoV-2 具有特别显著的竞争性抑制作用。在动物中预先感染肠道病毒会导致呼吸道中 SARS-CoV-2 病毒复制水平降低 100 倍以上，并从下呼吸道更快地清除传染性 SARS-CoV-2。SARS-CoV-2 与 LEV-8 或 EV-A71 的共同感染也降低了动物 SARS-CoV-2 感染的临床表现严重程度。此外，组织学数据表明，柯萨奇病毒 A7 的 LEV8 株的共同感染降低了 SARS-CoV-2 在肺部引起的病变水平。对趋化因子/细胞因子谱的研究表明，研究中的肠道病毒有效地触发了这部分抗病毒免疫反应，这与 SARS-CoV-2 感染的显著抑制有关。这些结果表明，在体外和体内，研究的柯萨奇病毒 A7 的 LEV-8 株或肠道病毒 A71 与 SARS-CoV-2 之间存在显著的病毒干扰。
