Weibel E R
Bull Eur Physiopathol Respir. 1979 Sep-Oct;15(5):999-1013.
This paper reviews the development of the methods for estimating morphometric parameters describing the human lung. The original work was done by light microscopy, but physiologically relevant data depend on studying the delicate structure of alveoli and capillaries by electron microscopy. Methodological improvements of recent years permitted this approach. This yielded estimates of alveolar surface which are by 80% larger than the original data; the reasons are explained by the gain in resolution. On the basis of these data, and by accounting for additional experimental information on the functional availability of gas exchanging surfaces, estimates of pulmonary diffusing capacity (DL) in the range of 90--190 ml O2 . min-1 . mmHg-1 are obtained, which compare reasonably well with physiological estimates of DL obtained under conditions of work.
本文综述了用于估计描述人类肺部形态计量学参数的方法的发展历程。最初的工作是通过光学显微镜完成的，但生理相关数据依赖于通过电子显微镜研究肺泡和毛细血管的精细结构。近年来的方法改进使得这种方法得以实现。这产生了比原始数据大80%的肺泡表面积估计值；分辨率的提高解释了其原因。基于这些数据，并考虑到关于气体交换表面功能可用性的额外实验信息，得到了90--190 ml O2·min-1·mmHg-1范围内的肺扩散容量（DL）估计值，这与工作条件下获得的DL生理估计值相当吻合。
