Fisher Carolyn D, Call Douglas R, Omulo Sylvia
Paul G. Allen School for Global Health, Washington State University, Pullman, WA, United States.
Washington State University Global Health-Kenya, Nairobi, Kenya.
Front Vet Sci. 2024 Jun 12;11:1294575. doi: 10.3389/fvets.2024.1294575. eCollection 2024.
Raw diets have become popular in companion animal nutrition, but these diets may be contaminated with harmful bacteria because heat processing is not utilized to mitigate pathogens during the production process. We analyzed 24 commercially available frozen raw canine and feline diets for extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E).
Samples were incubated in tryptic soy broth augmented with 50 μg/mL ampicillin to enrich for ESBL-E. ESBL-E were isolated using CHROMagar ESBL plates and isolate identification and antibiotic susceptibility testing were confirmed using the VITEK2 instrument.
ESBL-E were isolated from 42% (10/24) of raw diets, with complex and predominating. Most ESBL-E isolates (71%, 32/45) were multidrug-resistant. Direct plating of samples onto tryptic soy agar yielded bacterial counts >6 log for 2 samples from two different manufacturers.
This preliminary study justifies further investigation into the potential contribution of raw diets to the dissemination of antibiotic resistant bacteria in companion animals and domestic living spaces.
生食在伴侣动物营养中已变得流行，但由于在生产过程中未利用热处理来减轻病原体，这些饮食可能被有害细菌污染。我们分析了24种市售的冷冻生犬猫食品，以检测产超广谱β-内酰胺酶肠杆菌科细菌（ESBL-E）。
将样本在添加50μg/mL氨苄青霉素的胰蛋白胨大豆肉汤中培养，以富集ESBL-E。使用CHROMagar ESBL平板分离ESBL-E，并使用VITEK2仪器确认分离株鉴定和抗生素敏感性测试。
从42%（10/24）的生食中分离出ESBL-E，其中 复杂且占主导地位。大多数ESBL-E分离株（71%，32/45）对多种药物耐药。将两个不同制造商的2个样本直接接种到胰蛋白胨大豆琼脂上，细菌计数>6 log。
这项初步研究证明有必要进一步调查生食对伴侣动物和家庭生活空间中抗生素耐药菌传播的潜在影响。
