Romyasamit Chonticha, Sornsenee Phoomjai, Chimplee Siriphorn, Yuwalaksanakun Sitanun, Wongprot Dechawat, Saengsuwan Phanvasri
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand.
Department of Family and Preventive Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
PeerJ. 2021 Aug 25;9:e11787. doi: 10.7717/peerj.11787. eCollection 2021.
The increasing prevalence of broad-spectrum ampicillin-resistant and third-generation cephalosporin-resistant , particularly and , has become a global concern, with its clinical impacts on both human and veterinary medicine. This study examined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing and isolates from 10 types of raw vegetables.
In total, 305 samples were collected from 9 markets in Nakhon Si Thammarat, Thailand, in 2020.
ESBL-producing and isolates were found in 14 of the 305 samples obtained from 7 out of 10 types of vegetables (4.6% of the total). Further, 14 ESBL-producing ( = 5/14) and isolates ( = 9/14) (1.6% and 3.0%, respectively) were highly sensitive to β-lactam/carbapenem antibiotics (imipenem, 100%). ESBL-producing ( = 4) and isolates ( = 8) were also sensitive to non-β-lactam aminoglycosides (amikacin, 80.00% and 88.89%, respectively). ESBL producers were most resistant to β-lactam antibiotics, including ampicillin (85.71%) and the cephalosporins cefotaxime and ceftazidime (64.29%). The most frequently detected gene in ESBL-producing and was . However, two ESBL-producing isolates also carried three other ESBL-encoding variants, , , , which may be due to their association with food chains and humans.
Indeed, our results suggest that raw vegetables are an important source of ESBL-resistant and , which are potentially transmittable to humans via raw vegetable intake.
对广谱氨苄西林耐药和对第三代头孢菌素耐药，尤其是对 和 耐药的情况日益普遍，已成为一个全球关注的问题，对人类医学和兽医学都产生了临床影响。本研究调查了从10种生蔬菜中分离出的产超广谱β-内酰胺酶（ESBL）的 和 的流行情况、抗菌药敏性及分子遗传学特征。
2020年从泰国那空是贪玛叻府的9个市场共采集了305份样本。
在从10种蔬菜中的7种获取的305份样本中的14份中发现了产ESBL的 和 分离株（占总数的4.6%）。此外，14株产ESBL的 （ = 5/14）和 分离株（ = 9/14）（分别占1.6%和3.0%）对β-内酰胺/碳青霉烯类抗生素（亚胺培南，100%）高度敏感。产ESBL的 （ = 4）和 分离株（ = 8）对非β-内酰胺氨基糖苷类抗生素（阿米卡星，分别为80.00%和88.89%）也敏感。产ESBL菌株对β-内酰胺类抗生素耐药性最强，包括氨苄西林（85.71%）以及头孢菌素头孢噻肟和头孢他啶（64.29%）。在产ESBL的 和 中最常检测到的基因是 。然而，两株产ESBL的 分离株还携带了其他三种ESBL编码变体， 、 、 ，这可能是由于它们与食物链和人类的关联。
的确，我们的结果表明生蔬菜是耐ESBL的 和 的重要来源，它们有可能通过食用生蔬菜传播给人类。
