Chulay J D, Haynes J D, Diggs C L
Bull World Health Organ. 1985;63(2):317-23.
In vitro growth inhibition assays were used to detect antigenic differences among geographically distinct strains of Plasmodium falciparum. Owl monkeys were immunized against the Camp and FCR-3/FMG strains of P. falciparum by infection, drug treatment, and rechallenge with homologous parasites. Camp-immune monkey serum was used to inhibit the in vitro growth of eight strains of P. falciparum. Inhibition was maximum for the homologous Camp strain (an average of 62% inhibition by 100 ml/litre Camp-immune serum). Four other strains were inhibited to a lesser degree, and three strains (FCR-3/FMG, FVO, and Smith) were not significantly inhibited by Camp-immune serum at concentrations as high as 400 ml/litre. FCR-3/FMG-immune serum at a concentration of 50 ml/litre caused significant inhibition of the FCR-3/FMG strain, but not the Camp strain. Thus Camp and FCR-3/FMG strains appear to bear distinct antigenic determinants recognized by the homologous, but not the heterologous, antiserum. Inhibition of in vitro growth by immune serum may be useful for serotyping P. falciparum and may have application in the selection of strains for inclusion in a malaria vaccine.
采用体外生长抑制试验来检测恶性疟原虫不同地理株之间的抗原差异。通过感染、药物治疗以及用同源寄生虫再次攻击，对枭猴进行针对恶性疟原虫坎普（Camp）株和FCR - 3/FMG株的免疫。用坎普免疫猴血清抑制8种恶性疟原虫株的体外生长。对同源的坎普株抑制作用最大（100毫升/升的坎普免疫血清平均抑制率为62%）。其他4种菌株受到的抑制程度较小，而3种菌株（FCR - 3/FMG、FVO和史密斯株）在高达400毫升/升的浓度下未受到坎普免疫血清的显著抑制。浓度为50毫升/升的FCR - 3/FMG免疫血清对FCR - 3/FMG株有显著抑制作用，但对坎普株无抑制作用。因此，坎普株和FCR - 3/FMG株似乎带有同源抗血清能识别而异源抗血清不能识别的独特抗原决定簇。免疫血清对体外生长的抑制作用可能有助于恶性疟原虫的血清分型，并且可能在选择用于疟疾疫苗的菌株方面有应用价值。
