Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education & Key Laboratory of Medical Molecular Biology of Guizhou Province, Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry, Guizhou Medical University, Guiyang, Guizhou, China.
Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing, China.
Front Cell Infect Microbiol. 2024 Jun 14;14:1407051. doi: 10.3389/fcimb.2024.1407051. eCollection 2024.
The Cecum is a key site for cellulose digestion in nutrient metabolism of intestine, but its mechanisms of microbial and gene interactions has not been fully elucidated during pathogenesis of obesity. Therefore, the cecum tissues of the New Zealand rabbits and their contents between the high-fat diet-induced group (Ob) and control group (Co) were collected and analyzed using multi-omics. The metagenomic analysis indicated that the relative abundances of and species were significantly lower, while those of , , , , , , , and species were significantly higher in the Ob as compared to those in Co. Transcriptomic sequencing results showed that the differentially upregulated genes were mainly enriched in pathways, including calcium signaling pathway, PI3K-Akt signaling pathway, and Wnt signaling pathway, while the differentially downregulated genes were mainly enriched in pathways of NF-kappaB signaling pathway and T cell receptor signaling pathway. The comparative analysis of metabolites showed that the glycine, serine, and threonine metabolism and cysteine and methionine metabolism were the important metabolic pathways between the two groups. The combined analysis showed that , , and genes were highly correlated with , and species. Thus, the preliminary study elucidated the microbial and gene interactions in cecum of obese rabbit and provided a basis for further studies in intestinal intervention for human obesity.
盲肠是肠道营养代谢中纤维素消化的关键部位，但在肥胖发病机制中，其微生物和基因相互作用的机制尚未完全阐明。因此，本研究采用多组学方法收集并分析了高脂肪饮食诱导肥胖组（Ob）和对照组（Co）新西兰兔的盲肠组织及其内容物。宏基因组分析表明，与 Co 相比，Ob 中 和 物种的相对丰度显著降低，而 、 、 、 、 、 和 物种的相对丰度显著升高。转录组测序结果表明，差异上调基因主要富集在钙信号通路、PI3K-Akt 信号通路和 Wnt 信号通路等通路中，而差异下调基因主要富集在 NF-kappaB 信号通路和 T 细胞受体信号通路等通路中。代谢物的比较分析表明，甘氨酸、丝氨酸和苏氨酸代谢以及半胱氨酸和蛋氨酸代谢是两组之间的重要代谢途径。综合分析表明， 、 和 基因与 、 物种高度相关。因此，本初步研究阐明了肥胖兔盲肠中的微生物和基因相互作用，为进一步研究人类肥胖的肠道干预提供了基础。
