Liang Zhen-Pu, Wang Rui, Cao Han-Wen, Zhou Xin-Lu, Dai Jin-Ping, Su Ping, Singh Deepali, Zhang Xiao-Xia
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China.
Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi, 830000, China.
Arch Microbiol. 2025 Apr 3;207(5):112. doi: 10.1007/s00203-025-04315-1.
The strain Bachu38 was isolated from the rhizosphere soil of Tamarix chinensis Lour. plants in Bachu County, Xinjiang, China. This strain exhibited growth under a wide range of conditions, with temperature tolerance from 8 to 45°C (optimal at 28°C), pH adaptability between 7.0 and 10.0 (optimal range 8.0-9.0), and NaCl concentration tolerance spanning 2-20% (w/v), with optimal growth observed at 8% NaCl concentration. The cells displayed a spherical morphology and were Gram-positive, non-motile, and devoid of flagella. Biochemical characterization demonstrated positive reactions for both oxidase and catalase activities. Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain Bachu38 forms an independent branch within the genus Salinicoccus, sharing the highest sequence similarity with S. roseus DSM 5351 (98.43%) and S. amylolyticus JC304 (98.29%). The whole genome comprised 2.62 Mb, with a G + C content of 49.5%. When compared with the related strains, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 71.34-90.99% and 17.20-42.40%, respectively, which were below the suggested species identification threshold (ANI: < 95-96%, dDDH: < 70%) for different prokaryotic species. The respiratory quinone was identified as MK-6, and the cell wall peptidoglycan type was -Lys-Gly. Fatty acid analysis demonstrated that the major cellular fatty acids were iso-C and anteiso-C, each accounting for more than 10% of the total fatty acid composition. The polar lipid profile consisted of phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), glycolipid (GL), along with two unidentified lipids (designated as UL1 and UL2). Based on polyphasic taxonomic approaches incorporating phenotypic, physiological, and biochemical characteristics, along with comprehensive phylogenetic and genomic analyses, strain Bachu38 is proposed to represent a novel species of the genus Salinicoccus, named Salinicoccus bachuensis sp. nov., with strain Bachu38 (= CGMCC NO.28832 = JCM 37304) designated as the type strain.
菌株巴楚38是从中国新疆巴楚县柽柳植物的根际土壤中分离得到的。该菌株在广泛的条件下均能生长，耐受温度为8至45°C（最适温度为28°C），pH适应范围为7.0至10.0（最适范围8.0 - 9.0），NaCl浓度耐受范围为2 - 20%（w/v），在8% NaCl浓度下观察到最佳生长。细胞呈球形，革兰氏阳性，不运动，无鞭毛。生化特性表明氧化酶和过氧化氢酶活性均呈阳性反应。对16S rRNA基因序列的系统发育分析表明，菌株巴楚38在盐球菌属内形成一个独立分支，与玫瑰色盐球菌DSM 5351（98.43%）和淀粉分解盐球菌JC304（98.29%）的序列相似性最高。全基因组由2.62 Mb组成，G + C含量为49.5%。与相关菌株相比，平均核苷酸同一性（ANI）和数字DNA - DNA杂交（dDDH）值分别为71.34 - 90.99%和17.20 - 42.40%，均低于不同原核生物物种的建议物种鉴定阈值（ANI：< 95 - 96%，dDDH：< 70%）。呼吸醌被鉴定为MK - 6，细胞壁肽聚糖类型为-Lys - Gly。脂肪酸分析表明，主要细胞脂肪酸为异-C和anteiso - C，各占总脂肪酸组成的10%以上。极性脂质谱由磷脂酰甘油（PG）、二磷脂酰甘油（DPG）、糖脂（GL）以及两种未鉴定的脂质（命名为UL1和UL2）组成。基于多相分类方法，结合表型、生理和生化特征以及全面的系统发育和基因组分析，菌株巴楚38被提议代表盐球菌属的一个新物种，命名为巴楚盐球菌新种（Salinicoccus bachuensis sp. nov.），菌株巴楚38（= CGMCC NO.28832 = JCM 37304）被指定为模式菌株。
