Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Ecotoxicol Environ Saf. 2024 Sep 1;282:116692. doi: 10.1016/j.ecoenv.2024.116692. Epub 2024 Jul 5.
Viola yedoensis Makino (Vy) is a well-known traditional Chinese medicine widely used to treat inflammatory diseases. However, the regulatory effects of dietary Vy supplementation on lipopolysaccharide (LPS)-induced intestinal damage in broilers and the underlying molecular mechanisms remain unclear. In this study, broilers were intraperitoneally injected with 1 mg/kg LPS on days 17, 19 and 21 to induce intestinal damage. Vy supplementation at 0.5, 1.5 and 4.5 % in the diet was administered separately for 21 days to investigate the potential protective effects of Vy supplementation against LPS-induced intestinal impairment in broilers. Vy supplementation improved intestinal morphology and restored growth performance. Vy supplementation attenuated intestinal inflammation by regulating the nuclear factor kappa B (NF-κB) / NLR family pyrin domain-containing 3 (NLRP3) signaling pathway and inhibited its downstream pro-inflammatory factor levels. In addition, Vy supplementation relieved intestinal oxidative impairment by regulating the nuclear factor erythroid-2 related factor 2 (Nrf2) / mitogen-activated protein kinase (MAPK) signaling pathway and downstream antioxidant enzyme activity. Vy supplementation reduced LPS-induced mitochondrial damage and apoptosis. Furthermore, Vy supplementation alleviated LPS-induced intestinal inflammation and oxidative damage in chickens by increasing the abundance of protective bacteria (Lactobacillus and Romboutsia) and reducing the number of pathogenic bacteria (unclassified_f_Ruminococcaceae, unclassified_f_Oscillospiraceae and norank_f_norank_o_Clostridia_vadinBB60_group). Overall, Vy supplementation effectively ameliorated LPS-induced intestinal damage by regulating the NF-κB-NLRP3/Nrf2-MAPK signaling pathway and maintaining intestinal microbiota balance. Vy supplementation can be used as a dietary supplement to protect broilers against intestinal inflammation and oxidative damage.
野菰（Vy）是一种广为人知的传统中药，常用于治疗炎症性疾病。然而，饲粮添加野菰对 LPS 诱导的肉鸡肠道损伤的调控作用及其潜在的分子机制尚不清楚。本研究采用腹腔注射 LPS（1mg/kg）的方法在第 17、19 和 21 天诱导肉鸡肠道损伤，在饲粮中分别添加 0.5%、1.5%和 4.5%的野菰，连续添加 21 天，研究饲粮添加野菰对 LPS 诱导的肉鸡肠道损伤的潜在保护作用。结果表明，饲粮添加野菰改善了肉鸡肠道形态，恢复了生长性能。饲粮添加野菰通过调控核因子-κB（NF-κB）/NLR 家族pyrin 结构域包含 3（NLRP3）信号通路，抑制下游促炎因子水平，缓解了肠道炎症。此外，饲粮添加野菰通过调控核因子红细胞 2 相关因子 2（Nrf2）/丝裂原活化蛋白激酶（MAPK）信号通路及其下游抗氧化酶活性，缓解了肠道氧化损伤。饲粮添加野菰减轻了 LPS 诱导的肉鸡肠道线粒体损伤和凋亡。此外，饲粮添加野菰通过增加保护性细菌（乳杆菌和 Romboutsia）的丰度和降低致病性细菌（未分类_f_Ruminococcaceae、未分类_f_Oscillospiraceae 和 norank_f_norank_o_Clostridia_vadinBB60_group）的数量，缓解了 LPS 诱导的肉鸡肠道炎症和氧化损伤。综上所述，饲粮添加野菰通过调控 NF-κB-NLRP3/Nrf2-MAPK 信号通路和维持肠道微生物区系平衡，有效改善了 LPS 诱导的肉鸡肠道损伤。饲粮添加野菰可以作为一种饲料添加剂，保护肉鸡免受肠道炎症和氧化损伤。
