Malewicz B, Momsen M, Jenkin H M
Antimicrob Agents Chemother. 1983 Jan;23(1):119-24. doi: 10.1128/AAC.23.1.119.
Acyclovir, known as an antiherpetic agent, showed an inhibitory effect on the propagation of pseudorabies virus in BHK-21 cells. The antiviral effect of acyclovir was observed by plaque reduction, as well as by the inhibition of the virus-stimulated uptake of thymidine by BHK-21 cells. Amphotericin B potentiated the antiviral activity of acyclovir. The optimal concentrations of polyene antibiotic expressing the potentiating effect were lower than required for the induction of K+ leakage from the cells. There was no evident amphotericin B-induced stimulation of thymidine incorporation into infected BHK-21 cells. The model presented may be useful to study the potentiation phenomenon of polyene macrolide antibiotics.
阿昔洛韦作为一种抗疱疹剂，对伪狂犬病病毒在BHK - 21细胞中的增殖显示出抑制作用。通过蚀斑减少以及抑制病毒刺激的BHK - 21细胞摄取胸苷来观察阿昔洛韦的抗病毒效果。两性霉素B增强了阿昔洛韦的抗病毒活性。表现出增强作用的多烯抗生素的最佳浓度低于诱导细胞钾离子泄漏所需的浓度。两性霉素B对感染的BHK - 21细胞中胸苷掺入没有明显的刺激作用。所提出的模型可能有助于研究多烯大环内酯类抗生素的增强现象。
