Benítez J A, Alonso A, Delgado J, Kotyk A
Folia Microbiol (Praha). 1983;28(1):6-11. doi: 10.1007/BF02877377.
Sulphate uptake by Candida utilis follows Michaelis-Menten type kinetics characterized by a Km of 1.43 mM for sulphate. The process is unidirectional, pH, temperature and energy dependent. Molybdate, selenate, thiosulphate, chromate and sulphite are competitive inhibitors. Dithionite is a mixed-type inhibitor of sulphate uptake. If cells are pre-incubated with sulphate, sulphite, thiosulphate, dithionite or sulphide, sulphate uptake is severely blocked. Inhibition by endogenous sulphate, sulphite and thiosulphate was specific for sulphate uptake. Thus, incorporation of extracellular sulphate seems to be under the control of a heterogeneous pool of sulphur compounds. These results are discussed in connection with the regulation of sulphur amino acid biosynthesis in C. utilis.
产朊假丝酵母对硫酸盐的摄取遵循米氏动力学，其对硫酸盐的米氏常数为1.43 mM。该过程是单向的，依赖于pH、温度和能量。钼酸盐、硒酸盐、硫代硫酸盐、铬酸盐和亚硫酸盐是竞争性抑制剂。连二亚硫酸盐是硫酸盐摄取的混合型抑制剂。如果细胞预先用硫酸盐、亚硫酸盐、硫代硫酸盐、连二亚硫酸盐或硫化物孵育，硫酸盐摄取会受到严重阻碍。内源性硫酸盐、亚硫酸盐和硫代硫酸盐的抑制作用对硫酸盐摄取具有特异性。因此，细胞外硫酸盐的掺入似乎受硫化合物异质库的控制。结合产朊假丝酵母中硫氨基酸生物合成的调节对这些结果进行了讨论。
