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酿酒酵母中转录前 mRNA 剪接的剪接体介导的机制和调控。

Mechanisms and regulation of spliceosome-mediated pre-mRNA splicing in Saccharomyces cerevisiae.

机构信息

Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

出版信息

Wiley Interdiscip Rev RNA. 2024 Jul-Aug;15(4):e1866. doi: 10.1002/wrna.1866.

DOI:10.1002/wrna.1866
PMID:38972853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11585973/
Abstract

Pre-mRNA splicing, the removal of introns and ligation of flanking exons, is a crucial step in eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five small nuclear RNAs (snRNAs) and dozens of proteins, assembles on introns via a complex pathway before catalyzing the two transesterification reactions necessary for splicing. All of these steps have the potential to be highly regulated to ensure correct mRNA isoform production for proper cellular function. While Saccharomyces cerevisiae (yeast) has a limited set of intron-containing genes, many of these genes are highly expressed, resulting in a large number of transcripts in a cell being spliced. As a result, splicing regulation is of critical importance for yeast. Just as in humans, yeast splicing can be influenced by protein components of the splicing machinery, structures and properties of the pre-mRNA itself, or by the action of trans-acting factors. It is likely that further analysis of the mechanisms and pathways of splicing regulation in yeast can reveal general principles applicable to other eukaryotes. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Processing > Splicing Regulation/Alternative Splicing.

摘要

前体 mRNA 剪接，即内含子的去除和侧翼外显子的连接，是真核生物基因表达的关键步骤。剪接体是由五个小核 RNA（snRNA）和几十个蛋白质组成的大分子复合物，通过一个复杂的途径在内含子上组装，然后催化两个用于剪接的转酯化反应。所有这些步骤都有可能受到高度调控，以确保正确的 mRNA 亚型产生，从而实现细胞的正常功能。虽然酿酒酵母（酵母）只有有限数量的内含子基因，但其中许多基因的表达水平很高，导致细胞中大量的转录本被剪接。因此，剪接调控对酵母至关重要。就像在人类中一样，酵母剪接可以受到剪接机制的蛋白质成分、前体 mRNA 本身的结构和性质或反式作用因子的影响。进一步分析酵母剪接调控的机制和途径，可能会揭示适用于其他真核生物的一般原则。本文属于以下分类：RNA 加工 > 剪接机制 RNA 加工 > 剪接调控/可变剪接。

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Functional analysis of the zinc finger modules of the splicing factor Luc7.Luc7 剪接因子锌指模块的功能分析。
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2
Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strain.在人源化酵母菌株中，各个内含子对剪接抑制剂的反应存在广泛差异。
RNA. 2024 Jan 16;30(2):149-170. doi: 10.1261/rna.079866.123.
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Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.剪接因子 Prp18p 促进一致的 3'-剪接位点的全基因组保真度。
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Manipulating the 3D organization of the largest synthetic yeast chromosome.操控最大的合成酵母染色体的三维结构。
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5
RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing.Npl3p 通过 RNA 识别揭示了与剪接过程中伴侣蛋白功能兼容的 U2 snRNA 结合
Nat Commun. 2023 Nov 7;14(1):7166. doi: 10.1038/s41467-023-42962-4.
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Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor in .生化和遗传证据支持 Fyv6 作为. 的第二步剪接因子。
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