Wong Min Yi, Lin Bor-Shyh, Hu Po-Sheng, Huang Tsung-Yu, Huang Yao-Kuang
Division of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Puzi City, Chiayi County 61363, Taiwan.
College of Photonics, National Yang Ming Chiao Tung University, Tainan City 71150, Taiwan.
ACS Omega. 2024 Jun 18;9(26):28144-28154. doi: 10.1021/acsomega.4c01212. eCollection 2024 Jul 2.
Metal oxide nanoparticles with photothermal properties have attracted considerable research attention for their use in biomedical applications. Cesium tungsten oxide (CsWO) nanoparticles (NPs) exhibit strong absorption in the NIR region due to localized surface plasmon resonance, through which they convert light to heat; hence, they can be applied to photothermal treatment for bacteria and biofilm ablation. Herein, CsWO NPs were synthesized through solid-phase synthesis, and their physical properties were characterized through Zetasizer, energy dispersive X-ray spectroscopy, Fourier transform infrared spectrometer, and scanning and transmission electron microscopy (SEM and TEM, respectively). isolates were cultured in tryptic soy broth supplemented with glucose, and the biofilm inhibition and antibiofilm effects of the NPs were determined using a crystal violet assay and the Cell Counting Kit-8 (CCK-8) assay. The biofilm morphology and viability of NP-treated cultures after NIR irradiation were evaluated through SEM and confocal microscopy, respectively. The cytotoxicity of NPs to human macrophages was also assessed using the CCK-8 assay. The NPs effectively inhibited biofilm formation, with a formation rate of <10% and a viability rate of <50% at the concentration of ≥200 μg/mL. The confocal analysis revealed that NIR irradiation markedly enhanced biofilm cytotoxicity after treatment with the NPs. The assay of cytotoxicity to human macrophages demonstrated the biocompatibility of the NPs and NIR irradiation. In sum, the CsWO NPs displayed effective biofilm inhibition and antibiofilm activity at 200 μg/mL treatment concentration; they exhibited an enhancement effect under the NIR irradiation, suggesting CsWO NPs are a potential candidate agent for NIR-irradiated photothermal treatment in bacterial biofilm inhibition and antibiofilm.
具有光热性质的金属氧化物纳米颗粒因其在生物医学应用中的用途而吸引了大量研究关注。由于局域表面等离子体共振，铯钨氧化物（CsWO）纳米颗粒（NPs）在近红外区域表现出强烈吸收，通过这种吸收它们将光转化为热；因此，它们可应用于细菌和生物膜消融的光热处理。在此，通过固相合成法合成了CsWO NPs，并分别通过Zetasizer、能量色散X射线光谱仪、傅里叶变换红外光谱仪以及扫描和透射电子显微镜（分别为SEM和TEM）对其物理性质进行了表征。将分离株在补充有葡萄糖的胰蛋白胨大豆肉汤中培养，并使用结晶紫测定法和细胞计数试剂盒-8（CCK-8）测定法确定NPs的生物膜抑制和抗生物膜作用。分别通过SEM和共聚焦显微镜评估近红外照射后NP处理培养物的生物膜形态和活力。还使用CCK-8测定法评估了NPs对人巨噬细胞的细胞毒性。NPs有效抑制生物膜形成，在浓度≥200μg/mL时形成率<10%，存活率<50%。共聚焦分析表明，近红外照射显著增强了NP处理后的生物膜细胞毒性。对人巨噬细胞的细胞毒性测定证明了NPs和近红外照射的生物相容性。总之，CsWO NPs在200μg/mL处理浓度下显示出有效的生物膜抑制和抗生物膜活性；它们在近红外照射下表现出增强作用，表明CsWO NPs是细菌生物膜抑制和抗生物膜的近红外照射光热处理的潜在候选剂。
