Institut für Chemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
LOEWE-Centre for Translational Biodiversity Genomics, Senckenberganlage 25, 60325 Frankfurt am Main, Germany.
J Proteome Res. 2024 Aug 2;23(8):3524-3541. doi: 10.1021/acs.jproteome.4c00171. Epub 2024 Jul 9.
Snake venom variations are a crucial factor to understand the consequences of snakebite envenoming worldwide, and therefore it is important to know about toxin composition alterations between taxa. Palearctic vipers of the genera , , and have high medical impacts across the Old World. One hotspot for their occurrence and diversity is Türkiye, located on the border between continents, but many of their venoms remain still understudied. Here, we present the venom compositions of seven Turkish viper taxa. By complementary mass spectrometry-based bottom-up and top-down workflows, the venom profiles were investigated on proteomics and peptidomics level. This study includes the first venom descriptions of , , and , as well as the first snake venomics profiles of Turkish , and , including an in-depth reanalysis of venom. Additionally, we identified the modular consensus sequence pEXW(PZ)P(EI)/(KV)PPLE for bradykinin-potentiating peptides in viper venoms. For better insights into variations and potential impacts of medical significance, the venoms were compared against other Palearctic viper proteomes, including the first genus-wide venom comparison. This will help the risk assessment of snakebite envenoming by these vipers and aid in predicting the venoms' pathophysiology and clinical treatments.
蛇毒的变异是理解全球蛇伤中毒后果的一个关键因素，因此了解分类群之间毒素组成的变化非常重要。在旧世界，属、属、属和属的蝰蛇具有很高的医学影响。它们发生和多样性的一个热点地区是位于大陆边界的土耳其，但它们的许多毒液仍然研究不足。在这里，我们介绍了七种土耳其毒蛇的毒液成分。通过互补的基于质谱的自上而下和自下而上的工作流程，在蛋白质组学和肽组学水平上研究了毒液图谱。本研究包括对、、和的首次毒液描述，以及对土耳其、和的首次蛇毒组学图谱的描述，包括对 毒液的深入重新分析。此外，我们还确定了缓激肽增效肽的模块共识序列 pEXW(PZ)P(EI)/(KV)PPLE 在毒蛇毒液中。为了更好地了解变异和潜在的医学意义影响，将毒液与其他近东蝰蛇的蛋白质组进行了比较，包括首次对属进行的毒液比较。这将有助于评估这些蝰蛇引起的蛇伤中毒的风险，并有助于预测毒液的病理生理学和临床治疗。
