Institute of Nano-science and Nano-technology, College of Physical Science and Technology, Central China Normal University, Wuhan, Hubei 430079, China.
Hospital of Central China Normal University, Wuhan, Hubei 430079, China.
ACS Appl Mater Interfaces. 2024 Jul 24;16(29):37683-37697. doi: 10.1021/acsami.4c07092. Epub 2024 Jul 9.
Tissue engineering is theoretically considered a promising approach for repairing osteochondral defects. Nevertheless, the insufficient osseous support and integration of the cartilage layer and the subchondral bone frequently lead to the failure of osteochondral repair. Drawing from this, it was proposed that incorporating glycine-modified attapulgite (GATP) into poly(1,8-octanediol--citrate) (POC) scaffolds via the one-step chemical cross-linking is proposed to enhance cartilage and subchondral bone defect repair simultaneously. The effects of the GATP incorporation ratio on the physicochemical properties, chondrocyte and MC3T3-E1 behavior, and osteochondral defect repair of the POC scaffold were also evaluated. In vitro studies indicated that the POC/10% GATP scaffold improved cell proliferation and adhesion, maintained cell phenotype, and upregulated chondrogenesis and osteogenesis gene expression. Animal studies suggested that the POC/10% GATP scaffold has significant repair effects on both cartilage and subchondral bone defects. Therefore, the GATP-incorporated scaffold system with dual-lineage bioactivity showed potential application in osteochondral regeneration.
组织工程被理论上认为是一种有前途的修复方法，用于修复骨软骨缺损。然而，软骨层和软骨下骨的骨支持不足和整合常常导致骨软骨修复的失败。有鉴于此，有人提出通过一步化学交联将甘氨酸修饰的凹凸棒石（GATP）掺入聚（1,8-辛二醇-柠檬酸）（POC）支架中，以同时增强软骨和软骨下骨缺损的修复。还评估了 GATP 掺入比对 POC 支架的理化性质、软骨细胞和 MC3T3-E1 行为以及骨软骨缺损修复的影响。体外研究表明，POC/10%GATP 支架可提高细胞增殖和黏附性、维持细胞表型、上调软骨生成和成骨基因表达。动物研究表明，POC/10%GATP 支架对软骨和软骨下骨缺损均具有显著的修复作用。因此，具有双谱系生物活性的 GATP 掺入支架系统在骨软骨再生中具有潜在的应用前景。
