Smith Carlas, Kalisvaart Dylan, Prakash Kirti
Department of Mechanical Engineering, Delft Center for Systems and Control, Technische Universiteit Delft, Delft, Zuid-Holland, the Netherlands.
The Institute of Cancer Research, London, UK.
J Microsc. 2024 Nov;296(2):129-132. doi: 10.1111/jmi.13338. Epub 2024 Jul 10.
In single-molecule microscopy, a big question is how precisely we can estimate the location of a single molecule. Our research shows that by using iterative localisation microscopy and factoring in the prior information, we can boost precision and reduce the number of photons needed. Leveraging the Van Trees inequality aids in determining the optimal precision achievable. Our approach holds promise for wider application in discerning the optimal precision across diverse imaging scenarios, encompassing various illumination strategies, point spread functions and overarching control methodologies.
在单分子显微镜技术中，一个重要问题是我们能多精确地估计单个分子的位置。我们的研究表明，通过使用迭代定位显微镜并考虑先验信息，我们可以提高精度并减少所需的光子数量。利用范·特里不等式有助于确定可实现的最佳精度。我们的方法有望在更广泛的应用中辨别各种成像场景下的最佳精度，包括各种照明策略、点扩散函数和总体控制方法。
