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米蛾免疫和非免疫幼虫血淋巴对铜绿假单胞菌超微结构的影响

Effects of hemolymph from immune and non-immune larvae of Galleria mellonella on the ultra-structure of Pseudomonas aeruginosa.

作者信息

Chadwick J S, Deverno P J, Chung K L, Aston W P

出版信息

Dev Comp Immunol. 1982 Summer;6(3):433-40. doi: 10.1016/s0145-305x(82)80029-3.

Abstract

The ultrastructure of Pseudomonas aeruginosa, a pathogen of Galleria mellonella is rapidly altered after in vitro exposure to the hemolymph of vaccinated larvae. The bacteria were treated with normal and immune hemolymph for periods of time ranging from 7 to 28 min at 28 degrees C. In contrast to the apparent non-damaging effects of normal hemolymph, the immune hemolymph caused progressive damage to the cells within 7 min. The initial attack was directed towards the cell wall. Complete degradation was observed after 14 to 28 min exposure to the immune hemolymph.

摘要

绿脓杆菌是蜡螟的一种病原体,其超微结构在体外暴露于接种疫苗幼虫的血淋巴后会迅速改变。将细菌在28摄氏度下用正常血淋巴和免疫血淋巴处理7至28分钟。与正常血淋巴明显的无损伤作用相反,免疫血淋巴在7分钟内就对细胞造成了渐进性损伤。最初的攻击指向细胞壁。在暴露于免疫血淋巴14至28分钟后观察到完全降解。

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