Rasti Mehdi, Parniaei Amir Hossein, Dehghani Leila, Nasr Esfahani Salar, Mirhendi Hossein, Yazdani Vida, Azimian Zavareh Vajihe
Department of Plastic and Reconstructive Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Regen Ther. 2024 Jun 19;26:281-289. doi: 10.1016/j.reth.2024.06.004. eCollection 2024 Jun.
The skin plays a crucial role as a protective barrier against external factors, but disruptions to its integrity can lead to wound formation and hinder the natural healing process. Scar formation and delayed wound healing present significant challenges in skin injury treatment. While alternative approaches such as skin substitutes and tissue engineering exist, they are often limited in accessibility and cost. Exosomes have emerged as a potential solution for wound healing due to their regenerative properties.
In this study, exosomes were isolated from human blood serum using a kit. The exosomes were characterized, and their effects on cell migration were assessed . Additionally, the wound healing capacity of exosomes was evaluated using a rat full-thickness wound model.
Our findings revealed that exosomes significantly promoted cell migration. experiments demonstrated that the injection of exosomes at different areas of the wound accelerated the wound healing process, resulting in wound closure, collagen synthesis, vessel formation, and angiogenesis in the wound area. These results suggest that exosomes have a promising therapeutic potential for expediting wound healing and minimizing scar formation.
The findings of this study highlight the potential of exosomes as a novel approach for enhancing wound healing. Exosomes showed positive effects on both cell migration and wound closure in and studies, suggesting their potential use as a regenerative therapy for skin injuries. Further research is needed to fully understand the mechanisms underlying the beneficial effects of exosomes on wound healing and to optimize their application in clinical settings.
皮肤作为抵御外部因素的保护屏障起着至关重要的作用，但皮肤完整性的破坏会导致伤口形成并阻碍自然愈合过程。瘢痕形成和伤口愈合延迟在皮肤损伤治疗中带来了重大挑战。虽然存在皮肤替代物和组织工程等替代方法，但它们在可及性和成本方面往往受到限制。由于其再生特性，外泌体已成为伤口愈合的一种潜在解决方案。
在本研究中，使用试剂盒从人血清中分离外泌体。对外泌体进行了表征，并评估了它们对细胞迁移的影响。此外，使用大鼠全层伤口模型评估了外泌体的伤口愈合能力。
我们的研究结果表明外泌体显著促进细胞迁移。实验表明，在伤口的不同区域注射外泌体加速了伤口愈合过程，导致伤口闭合、胶原蛋白合成、血管形成以及伤口区域的血管生成。这些结果表明外泌体在加速伤口愈合和最小化瘢痕形成方面具有有前景的治疗潜力。
本研究结果突出了外泌体作为一种促进伤口愈合的新方法的潜力。外泌体在[具体实验]和[具体实验]研究中对细胞迁移和伤口闭合均显示出积极作用，表明它们作为皮肤损伤再生疗法的潜在用途。需要进一步研究以充分了解外泌体对伤口愈合有益作用的潜在机制，并优化它们在临床环境中的应用。
