Whiteside J P, Farmer S, Spier R E
Dev Biol Stand. 1985;60:283-90.
A simple, unaerated 0.1 litre microcarrier propagator was successfully sealed up to 1 litre using constant tip speed of the flexible wide blade stirrer. Further scale-up to 10 litres was not successful because of inadequate aeration. When this deficiency was corrected, using a sintered aerator, cell growth did not improve due to excessive foam production. This was overcome by carrying out the necessary aeration inside a special cage, located inside the propagator, which retained the bubbles and excluded the microcarriers. Using this device cell growth improved to levels similar to those obtained at the 0.1 and 1 litre scales.
一个简单的、未通气的0.1升微载体增殖器，通过灵活宽叶片搅拌器的恒定叶尖速度成功扩大到了1升。由于通气不足，进一步扩大到10升未成功。当使用烧结曝气器纠正这一缺陷时，由于产生过多泡沫，细胞生长并未改善。在增殖器内放置一个特殊的笼子，在笼子内部进行必要的通气，该笼子能留住气泡并排除微载体，从而克服了这一问题。使用该装置，细胞生长改善到了与0.1升和1升规模时相似的水平。
