大肠杆菌二氢叶酸还原酶缺陷型突变体的分离
Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.
作者信息
Singer S, Ferone R, Walton L, Elwell L
出版信息
J Bacteriol. 1985 Oct;164(1):470-2. doi: 10.1128/jb.164.1.470-472.1985.
Abstract
A strain of Escherichia coli was isolated in which dihydrofolate reductase was not detected by an enzyme assay or by competition for antibody. This strain requires methionine, glycine, a purine, and thymidine for growth in addition to the auxotrophic requirements of the parent strain. It was found to be useful as a recipient of plasmids harboring dihydrofolate reductase genes.
摘要
分离出了一株大肠杆菌，通过酶测定或抗体竞争法均未检测到其二氢叶酸还原酶。除了亲代菌株的营养缺陷型需求外，该菌株生长还需要甲硫氨酸、甘氨酸、一种嘌呤和胸腺嘧啶核苷。发现它作为携带二氢叶酸还原酶基因的质粒的受体很有用。
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