State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650091, China.
Int J Mol Sci. 2024 Jul 5;25(13):7384. doi: 10.3390/ijms25137384.
The capsule-associated protein 10 gene () is indispensable due to its involvement in pod formation and virulence maintenance in . The function of the gene in nematode-predatory fungi remains unreported. As a typical nematode-trapping fungus, efficiently captures nematodes using adhesive knobs, which has potential applications in the biological control of plant-parasitic nematodes. In this study, we investigated the function of DHXT1 (a CAP10 homologous protein) in -nematode interactions based on the disruption and overexpression of , phenotypic analysis and metabolomic analysis. As a result, it was shown that the disruption of the gene causes a marked decrease in the number of adhesive knobs, and on the contrary, the overexpression of the gene causes a substantial increase in the number of adhesive knobs. Interestingly, the variety of metabolites increased with the disruption of the and decreased with the overexpression of the gene. The results suggest that effects pathogenicity through its involvement in adhesive knobs' formation and metabolite synthesis and serves as a key virulence factor in .
衣壳相关蛋白 10 基因 () 对于形成荚膜和维持毒力至关重要。在食线虫真菌中， 基因的功能尚未报道。作为一种典型的食线虫真菌， 利用粘性节结高效捕获线虫，在植物寄生线虫的生物防治中有潜在的应用。在这项研究中，我们基于 的敲除和过表达，通过表型分析和代谢组学分析，研究了 DHXT1（CAP10 同源蛋白）在 -线虫相互作用中的功能。结果表明， 基因的敲除导致粘性节结数量明显减少，而 基因的过表达则导致粘性节结数量大量增加。有趣的是，随着 基因的敲除，代谢物的种类增加，而随着 基因的过表达，代谢物的种类减少。这些结果表明， 通过参与粘性节结的形成和代谢物的合成影响致病性，并作为 的关键毒力因子。
