使用聚乙二醇在经放线菌酮处理的 McCoy 细胞中分离和培养沙眼衣原体的改良方法。
Improved method for isolation and growth of Chlamydia trachomatis in McCoy cells treated with cycloheximide using polyethylene glycol.
作者信息
Mohammed N R, Hillary I B
出版信息
J Clin Pathol. 1985 Sep;38(9):1052-4. doi: 10.1136/jcp.38.9.1052.
Abstract
The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critical. A concentration of 35% polyethylene glycol in barbitone added to the cell culture growth medium either immediately before or immediately after infection with chlamydia increased the number of inclusions detected. The rate of isolation obtained from clinical specimens was also increased when cell cultures treated with polyethylene glycol were used.
摘要
将用聚乙二醇预处理的非复制性 McCoy 细胞用于从临床标本中分离沙眼衣原体以及实验室菌株生长的敏感性，与未处理的非复制性细胞培养物的敏感性进行了比较。不同溶液中聚乙二醇的浓度以及添加到细胞培养基中的时间至关重要。在感染衣原体之前或之后立即将巴比妥中 35% 的聚乙二醇浓度添加到细胞培养生长培养基中，可增加检测到的包涵体数量。当使用经聚乙二醇处理的细胞培养物时，从临床标本中获得的分离率也有所提高。
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本文引用的文献
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