用于检测临床标本中沙眼衣原体的HeLa 229细胞培养物与McCoy细胞培养物的比较。
Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
作者信息
Thewessen E A, Freundt I, van Rijsoort-Vos J H, Stolz E, Michel M F, Wagenvoort J H
机构信息
Department of Clinical Microbiology, University Hospital Rotterdam, The Netherlands.
出版信息
J Clin Microbiol. 1989 Jun;27(6):1399-400. doi: 10.1128/jcm.27.6.1399-1400.1989.
Abstract
Consecutive clinical specimens of Chlamydia trachomatis (1,048) were inoculated in parallel on DEAE-dextran- and cycloheximide-treated HeLa 229 cells and cycloheximide-treated McCoy cells. HeLa 229 cell culture detected 113 positive specimens, and McCoy cell culture detected 103 positive specimens. This difference is not significant. However, HeLa 229 cell culture yielded significantly more inclusions than McCoy cell culture in the 95 specimens positive in both cell types (P = 0.042). For routine diagnostic purposes, a choice for one of the cell types may be determined by local preferences.
摘要
将1048份沙眼衣原体连续临床标本同时接种于经二乙氨基乙基葡聚糖（DEAE - 葡聚糖）和放线菌酮处理的HeLa 229细胞以及经放线菌酮处理的McCoy细胞。HeLa 229细胞培养检测出113份阳性标本，McCoy细胞培养检测出103份阳性标本。这种差异不显著。然而，在两种细胞类型均为阳性的95份标本中，HeLa 229细胞培养产生的包涵体明显多于McCoy细胞培养（P = 0.042）。出于常规诊断目的，可根据当地偏好来决定选择其中一种细胞类型。
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