Thomas B J, Evans R T, Hutchinson G R, Taylor-Robinson D
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.
Detection of Chlamydia trachomatis inclusions only 21 h after a specimen reaches the laboratory has been achieved by the combined use of cycloheximide-treated McCoy cells and immunofluorescence staining. Moreover, cells exposed to cycloheximide were more sensitive for detecting chlamydial inclusions than those pretreated by irradiation, since larger numbers of inclusions were found in the former cells. The application of this rapid and sensitive method allows a diagnosis of chlamydial infection to be made before antibiotic therapy is started. In this way, it should enable the treatment of nonspecific genital infections to be placed on a more rational basis.
通过联合使用经放线菌酮处理的 McCoy 细胞和免疫荧光染色,在标本送达实验室仅 21 小时后就能检测到沙眼衣原体包涵体。此外,与经辐射预处理的细胞相比,接触放线菌酮的细胞在检测衣原体包涵体方面更敏感,因为在前者细胞中发现了更多的包涵体。这种快速灵敏方法的应用使得在开始抗生素治疗之前就能做出衣原体感染的诊断。这样,应该能够使非特异性生殖器感染的治疗建立在更合理的基础上。