沙眼衣原体分离的微量试验程序。
Microtest procedure for isolation of Chlamydia trachomatis.
作者信息
Yoder B L, Stamm W E, Koester C M, Alexander E R
出版信息
J Clin Microbiol. 1981 Jun;13(6):1036-9. doi: 10.1128/jcm.13.6.1036-1039.1981.
Abstract
Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a microtiter method that exhibited equal sensitivity but less frequent contamination than our previously used vial-cover slip culture system. In addition, costs and technician time were substantially reduced with the microtest method. Subsequent studies showed that cycloheximide-treated cells were superior to 5-iodo-2-deoxyuridine-treated cells in the microtest method and that a subpassage significantly enhanced the sensitivity of the method. The microtest method appears to be a sensitive, rapid, and economical method for isolating C. trachomatis.
摘要
沙眼衣原体潜在感染患者的管理一直受到衣原体培养所需成本和时间的阻碍。为了分离沙眼衣原体，我们开发了一种微量滴定法，该方法具有同等的敏感性，但污染频率低于我们之前使用的小瓶-盖玻片培养系统。此外，微量试验法大大降低了成本和技术人员的时间。后续研究表明，在微量试验法中，经环己酰亚胺处理的细胞优于经5-碘-2-脱氧尿苷处理的细胞，并且传代显著提高了该方法的敏感性。微量试验法似乎是一种分离沙眼衣原体敏感、快速且经济的方法。
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