Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Chengdu University, Chengdu 610106, China; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Chongqing Academy of Animal Sciences, Chongqing 402460, China.
Sci Total Environ. 2024 Oct 15;947:174734. doi: 10.1016/j.scitotenv.2024.174734. Epub 2024 Jul 14.
The ongoing and progressive evolution of antibiotic resistance presents escalating challenges for the clinical management and prevention of bacterial infections. Understanding the makeup of resistance genomes and accurately quantifying the current abundance of antibiotic resistance genes (ARGs) are crucial for assessing the threat of antimicrobial resistance (AMR) to public health. This comprehensive study investigated the distribution and diversity of bacterial community composition, ARGs, and virulence factors (VFs) across human, chicken, pig fecal, and soil microbiomes in various provinces of China. As a result, multidrug resistance was identified across all samples. Core ARGs primarily related to multidrug, MLS (Macrolides-Lincosamide-Streptogramins), and tetracycline resistance were characterized. A significant correlation between ARGs and bacterial taxa was observed, especially in soil samples. Probiotic strains such as Lactobacillus harbored ARGs, potentially contributing to the dissemination of antibiotic resistance. We screened subsets of ARGs from samples from different sources as indicators to assess the level of ARGs contamination in samples, with high accuracy. These results underline the complex relationship between microbial communities, resistance mechanisms, and environmental factors, emphasizing the importance of continued research and monitoring to better understand these dynamics.
抗生素耐药性的持续和不断发展，对细菌感染的临床管理和预防提出了日益严峻的挑战。了解耐药基因组的构成，并准确量化当前抗生素耐药基因 (ARGs) 的丰度，对于评估抗菌药物耐药性 (AMR) 对公众健康的威胁至关重要。本研究全面调查了中国不同省份的人、鸡、猪粪便和土壤微生物组中细菌群落组成、ARGs 和毒力因子 (VF) 的分布和多样性。结果显示，所有样本均存在多种耐药性。主要与多药、MLS（大环内酯类-林可酰胺类-链阳菌素类）和四环素耐药相关的核心 ARGs 得到了表征。观察到 ARGs 与细菌分类群之间存在显著相关性，尤其是在土壤样本中。益生菌菌株如乳杆菌携带 ARGs，可能有助于抗生素耐药性的传播。我们从不同来源的样本中筛选了 ARGs 亚组作为指示物，以评估样本中 ARGs 污染的水平，具有很高的准确性。这些结果强调了微生物群落、耐药机制和环境因素之间的复杂关系，强调了持续研究和监测的重要性，以更好地了解这些动态。
