Nagy G, Mezey I
Acta Microbiol Acad Sci Hung. 1977;24(3):189-94.
IgM and IgG immunoglobulins of human sera were separated by stepwise column chromatography in QAE-Sephadex A-25 ion exchanger gel bed. The procedure resulted within 30 min in a fraction suitable for direct titration of rubella-specific IgM antibodies by haemagglutination inhibition test. The method proved to be a useful diagnostic tool for primary rubella. Serum samples of 13 individuals with previously acquired immunity, 152 patients with a recent rubella-like illness, and 194 pregnant women exposed to rubella infection were tested for the presence of rubella-specific IgM antibodies. Sera of individuals with previous immunity proved to be negative for specific IgM antibodies. Specific IgM titre was demonstrated in the blood of all the 25 patients with significant titre-rise tested because of rubella-like illness, and also in the sera of additional 8 patients whose serum samples were taken too late for demonstration of a rise in titre. Significant titre-rises were found in 5 women exposed to rubella infection, but only two of them exhibited rubella-specific IgM antibodies. The absence of specific IgM antibodies refers presumably to subclinical reinfection in the other three cases.
通过在QAE-葡聚糖A-25离子交换剂凝胶床中进行分步柱色谱法分离人血清中的IgM和IgG免疫球蛋白。该方法在30分钟内得到适合通过血凝抑制试验直接滴定风疹特异性IgM抗体的组分。该方法被证明是原发性风疹的一种有用诊断工具。对13名具有既往获得性免疫力的个体、152名近期患风疹样疾病的患者以及194名暴露于风疹感染的孕妇的血清样本进行了风疹特异性IgM抗体检测。具有既往免疫力的个体血清中特异性IgM抗体检测为阴性。在所有因风疹样疾病而检测的25名滴度显著升高的患者血液中以及另外8名血清样本采集时间过晚无法显示滴度升高的患者血清中均检测到特异性IgM滴度。在5名暴露于风疹感染的女性中发现了显著的滴度升高，但其中只有两名表现出风疹特异性IgM抗体。另外三例中未检测到特异性IgM抗体可能是由于亚临床再感染。
