Ali Nazaridoost, Nastaran Ansari, Ali Teimoori, Farid Azizi Jalilian
Department of Medical Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Niger Med J. 2024 Apr 21;65(1):31-39. doi: 10.60787/nmj-v65i1-449. eCollection 2024 Jan-Feb.
The presence of other respiratory pathogens in patients with SARS-CoV-2 infection has been described as a striking feature. However, data on adenovirus co-infection rates and clinical impacts in COVID-19 patients is limited. The purpose of this study is to compare the prevalence of respiratory adenoviruses in children under 15 years of age in Positive and Negative SARS-CoV-2 patients.
From September 2020 to January 2021, nasopharyngeal swabs were obtained from 280 patients below 15 years old with influenza-like infection symptoms suspected to be COVID-19 and referred to hospitals in Hamadan province. Nucleic acid was extracted using a High Pure Viral Nucleic acid extraction kit for both viral RNA and DNA. Reverse transcription real-time PCR for detecting SARS-CoV-2 and Real-time PCR for Human Adenoviruses were used.
Out of 280 examined samples, 11.7% tested positive for AdV, of which 18 samples originated from the SARS-CoV-2 positive group and 15 samples were from the SARS-CoV-2 negative group. Of 18 co-infected samples, which were categorized in three different ranges of age including, 0-5, 6-10, and 11-15 years old were 11, 4, and 3 patients respectively. Also, 14 patients were hospitalized. Compared with AdV-positive patients, children with Co-infection with SARS CoV-2 had lower levels of white blood cell (WBC) count while erythrocyte sedimentation rate (ESR) and C-reaction protein (CRP) had increased levels.
We report a substantial burden of AdV co-infection in pediatric COVID-19 patients. This study revealed most AdV infections lead to hospitalization and change in parameters.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染患者中存在其他呼吸道病原体是一个显著特征。然而，关于腺病毒合并感染率及其对2019冠状病毒病（COVID-19）患者临床影响的数据有限。本研究的目的是比较SARS-CoV-2检测呈阳性和阴性的15岁以下儿童中呼吸道腺病毒的流行情况。
2020年9月至2021年1月，从哈马丹省医院收治的280例疑似COVID-19的15岁以下流感样感染症状患者中采集鼻咽拭子。使用高纯病毒核酸提取试剂盒提取病毒RNA和DNA的核酸。采用逆转录实时荧光定量PCR检测SARS-CoV-2，实时荧光定量PCR检测人腺病毒。
在280份检测样本中，11.7%的样本腺病毒检测呈阳性，其中18份样本来自SARS-CoV-2阳性组，15份样本来自SARS-CoV-2阴性组。在18例合并感染的样本中，按年龄分为0-5岁、6-10岁和11-15岁三个不同范围，分别有11例、4例和3例患者。此外，14例患者住院治疗。与腺病毒阳性患者相比，合并感染SARS-CoV-2的儿童白细胞（WBC）计数较低，而红细胞沉降率（ESR）和C反应蛋白（CRP）水平升高。
我们报告了儿童COVID-19患者中腺病毒合并感染的沉重负担。本研究显示，大多数腺病毒感染导致住院治疗及参数变化。
