Long Lijuan, Bi Hongjuan, Zeng Shangjuan, Wang Shuangjie, Zhang Zhen, Yao Jiayan, Wang Zhiping
Department of Neonatology, Guangxi Zhuang Autonomous Region Maternal and Child Health Hospital Nanning, Guangxi Zhuang Autonomous Region, China.
Graduate School, Guangxi Medical University Nanning, Guangxi Zhuang Autonomous Region, China.
Am J Transl Res. 2024 Jun 15;16(6):2474-2482. doi: 10.62347/IJMS4900. eCollection 2024.
To determine whether and how breast feeding of premature infants influences the human milk (HM) bacterial communities.
HM samples before and after breastfeeding were collected from 40 preterm infant mothers at 24-36 weeks of gestational age in the neonatal intensive care unit of our hospital. Of these 40 babies, 11 at 24-27 weeks of gestational age and 12 at 28-31 weeks were grouped into an extremely premature (EPM) group and a very premature (VPM) group, respectively. In addition, 11 with a birth weight (BWT) of 1000 g ≤ BWT < 1500 g were classified as a very low birth weight (VLBW) group and 12 with BWT < 1000 g an extremely low birth weight (ELBW) group. Breast feeding and kangaroo mother care were given once a day for 7 days, from 14 to 21 days of age. The bacterial composition of HM was analyzed using high-throughput sequencing before and after feeding.
Linear discriminant analysis effect size of HM samples before and after feeding showed that and were significantly enriched in HM before breastfeeding (P < 0.05). Post-feeding HM for the EPM group showed significant enrichment in and (P < 0.05). was significantly enriched in the HM for EPM group before feeding (P < 0.05). For mothers with VLBW infants, was enriched before feeding, while was predominant after feeding (P < 0.05). There was a moderate correlation between the diversity of HM bacteria and infant development and immune outcomes.
Breastfeeding of preterm infants can significantly affect the bacterial diversity in HM.
确定早产儿母乳喂养是否以及如何影响人乳（HM）细菌群落。
在我院新生儿重症监护病房，收集了40例孕24 - 36周早产儿母亲母乳喂养前后的HM样本。这40例婴儿中，孕24 - 27周的11例和孕28 - 31周的12例分别被分为极早产儿（EPM）组和早产儿（VPM）组。此外，出生体重（BWT）为1000 g≤BWT＜1500 g的11例被归为极低出生体重（VLBW）组，BWT＜1000 g的12例为超低出生体重（ELBW）组。在婴儿14至21日龄时，每天进行一次母乳喂养和袋鼠式护理，持续7天。喂养前后采用高通量测序分析HM的细菌组成。
喂养前后HM样本的线性判别分析效应大小显示，[具体细菌名称1]和[具体细菌名称2]在母乳喂养前的HM中显著富集（P＜0.05）。EPM组喂养后的HM中[具体细菌名称3]和[具体细菌名称4]显著富集（P＜0.05）。[具体细菌名称5]在EPM组喂养前的HM中显著富集（P＜0.05）。对于VLBW婴儿的母亲，[具体细菌名称6]在喂养前富集，而[具体细菌名称7]在喂养后占主导（P＜0.05）。HM细菌多样性与婴儿发育和免疫结局之间存在中度相关性。
早产儿母乳喂养可显著影响HM中的细菌多样性。
