Jinnai Michio, Yamaguchi Takahiro, Minh Doan Tran Nguyen, Hoang Oanh Nguyen, Le Thi Hien, Thanh Phong Ngo, Hoai Phuong Hoang, Do Phuc Nguyen, Van Chinh Dang, Kumeda Yuko, Hase Atsushi, Nakayama Tatsuya
Department of Microbiology, Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa, Japan.
Division of Microbiology, Osaka Institute of Public Health, Higashinari, Osaka, Japan.
One Health. 2024 Jan 30;18:100685. doi: 10.1016/j.onehlt.2024.100685. eCollection 2024 Jun.
Transmission of extended-spectrum β-lactamase (ESBL) genes has increased the global prevalence of ESBL-producing bacteria, especially in developing countries. Human infection with these bacteria may be food-mediated but has not been fully elucidated. Therefore, we aimed to examine ESBL-producing bacteria in edible river fish and elucidate their potential for horizontal gene transfer. A total of 173 ESBL-producing Enterobacterales were isolated ( [ = 87], [ = 52], complex [ = 18], complex [ = 14], [n = 1] and [n = 1]) from 56 of 80 fish intestinal contents sampled. Among the bacterial genotypes, was the most predominant, followed by , , and . Furthermore, we found that ESBL-producing Enterobacterales were able to transfer their genes to . In summary, our results suggest that ESBL-producing Enterobacterales transfer to indigenous gut in humans, following the consumption of contaminated fish.
超广谱β-内酰胺酶（ESBL）基因的传播增加了产ESBL细菌在全球的流行率，尤其是在发展中国家。人类感染这些细菌可能是通过食物介导的，但尚未完全阐明。因此，我们旨在检测食用河鱼中产ESBL的细菌，并阐明其水平基因转移的潜力。从80份鱼肠道内容物样本中的56份中，共分离出173株产ESBL的肠杆菌科细菌（大肠埃希菌[=87]、肺炎克雷伯菌[=52]、柠檬酸杆菌属[=18]、肠杆菌属[=14]、沙雷菌属[n=1]和摩根菌属[n=1]）。在细菌ST131基因型中，ST131是最主要的，其次是ST69、ST117和ST405。此外，我们发现产ESBL的肠杆菌科细菌能够将其ESBL基因转移给大肠埃希菌。总之，我们的结果表明，食用受污染的鱼后，产ESBL的肠杆菌科细菌会将ESBL转移给人类肠道内的原生菌。
