Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.
Influenza Other Respir Viruses. 2024 Jul;18(7):e13339. doi: 10.1111/irv.13339.
We describe humoral immune responses in 105 ambulatory patients with laboratory-confirmed SARS-CoV-2 Omicron variant infection. In dried blood spot (DBS) collected within 5 days of illness onset and during convalescence, we measured binding antibody (bAb) against ancestral spike protein receptor binding domain (RBD) and nucleocapsid (N) protein using a commercial multiplex bead assay. Geometric mean bAb concentrations against RBD increased by a factor of 2.5 from 1258 to 3189 units/mL and by a factor of 47 against N protein from 5.5 to 259 units/mL between acute illness and convalescence; lower concentrations were associated with greater geometric mean ratios. Paired DBS specimens may be used to evaluate humoral response to SARS-CoV-2 infection.
我们描述了 105 例经实验室确诊的 SARS-CoV-2 奥密克戎变异株感染的门诊患者的体液免疫反应。在发病后 5 天内和恢复期采集的干血斑 (DBS) 中，我们使用商业多重珠粒分析测定了针对原始刺突蛋白受体结合域 (RBD) 和核衣壳 (N) 蛋白的结合抗体 (bAb)。与急性疾病相比，恢复期 RBD 的 bAb 浓度从 1258 单位/毫升增加到 3189 单位/毫升，增加了 2.5 倍；针对 N 蛋白的 bAb 浓度从 5.5 单位/毫升增加到 259 单位/毫升，增加了 47 倍；较低的浓度与更高的几何平均比值相关。配对的 DBS 标本可用于评估针对 SARS-CoV-2 感染的体液免疫反应。
